mRNA encoding 'ClC-K1, a kidney Cl-- channel' is expressed in marginal cells of the stria vascularis of rat cochlea: Its possible contribution to Cl- currents

Motonori Ando, Shunji Takeuchi

Research output: Contribution to journalArticle

28 Citations (Scopus)

Abstract

The cochlear stria vascularis is essential for the normal function of hair cells. mRNA encoding the ClC-K1 Cl- channel, previously thought to be found only in the kidney, was detected in epithelial marginal cells of the rat stria vascularis by single cell RT-PCR. When Cl- currents were recorded from rat marginal cells by the whole-cell patch clamp method, the steady-state currents showed weak outward rectification and an ion selectivity sequence of SCN->Br-=Cl->F->NO3->I->gluconate-. The Cl- currents were regulated by extracellular Ca2+ and pH. These characteristics resemble those reported for the currents recorded from Xenopus oocytes expressing ClC-K1 Cl- channels. These data together suggest that ClC-K1 Cl- channels may contribute to the whole-cell currents of marginal cells. Copyright (C) 2000 Elsevier Science Ireland Ltd.

Original languageEnglish
Pages (from-to)171-174
Number of pages4
JournalNeuroscience Letters
Volume284
Issue number3
DOIs
Publication statusPublished - Apr 28 2000
Externally publishedYes

Fingerprint

Stria Vascularis
Cochlea
Kidney
Messenger RNA
Xenopus
Oocytes
Epithelial Cells
Ions
Polymerase Chain Reaction

Keywords

  • Endolymph
  • Inner ear
  • Ion transport
  • Patch clamp
  • Single cell RT-PCR

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

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title = "mRNA encoding 'ClC-K1, a kidney Cl-- channel' is expressed in marginal cells of the stria vascularis of rat cochlea: Its possible contribution to Cl- currents",
abstract = "The cochlear stria vascularis is essential for the normal function of hair cells. mRNA encoding the ClC-K1 Cl- channel, previously thought to be found only in the kidney, was detected in epithelial marginal cells of the rat stria vascularis by single cell RT-PCR. When Cl- currents were recorded from rat marginal cells by the whole-cell patch clamp method, the steady-state currents showed weak outward rectification and an ion selectivity sequence of SCN->Br-=Cl->F->NO3->I->gluconate-. The Cl- currents were regulated by extracellular Ca2+ and pH. These characteristics resemble those reported for the currents recorded from Xenopus oocytes expressing ClC-K1 Cl- channels. These data together suggest that ClC-K1 Cl- channels may contribute to the whole-cell currents of marginal cells. Copyright (C) 2000 Elsevier Science Ireland Ltd.",
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T1 - mRNA encoding 'ClC-K1, a kidney Cl-- channel' is expressed in marginal cells of the stria vascularis of rat cochlea

T2 - Its possible contribution to Cl- currents

AU - Ando, Motonori

AU - Takeuchi, Shunji

PY - 2000/4/28

Y1 - 2000/4/28

N2 - The cochlear stria vascularis is essential for the normal function of hair cells. mRNA encoding the ClC-K1 Cl- channel, previously thought to be found only in the kidney, was detected in epithelial marginal cells of the rat stria vascularis by single cell RT-PCR. When Cl- currents were recorded from rat marginal cells by the whole-cell patch clamp method, the steady-state currents showed weak outward rectification and an ion selectivity sequence of SCN->Br-=Cl->F->NO3->I->gluconate-. The Cl- currents were regulated by extracellular Ca2+ and pH. These characteristics resemble those reported for the currents recorded from Xenopus oocytes expressing ClC-K1 Cl- channels. These data together suggest that ClC-K1 Cl- channels may contribute to the whole-cell currents of marginal cells. Copyright (C) 2000 Elsevier Science Ireland Ltd.

AB - The cochlear stria vascularis is essential for the normal function of hair cells. mRNA encoding the ClC-K1 Cl- channel, previously thought to be found only in the kidney, was detected in epithelial marginal cells of the rat stria vascularis by single cell RT-PCR. When Cl- currents were recorded from rat marginal cells by the whole-cell patch clamp method, the steady-state currents showed weak outward rectification and an ion selectivity sequence of SCN->Br-=Cl->F->NO3->I->gluconate-. The Cl- currents were regulated by extracellular Ca2+ and pH. These characteristics resemble those reported for the currents recorded from Xenopus oocytes expressing ClC-K1 Cl- channels. These data together suggest that ClC-K1 Cl- channels may contribute to the whole-cell currents of marginal cells. Copyright (C) 2000 Elsevier Science Ireland Ltd.

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KW - Inner ear

KW - Ion transport

KW - Patch clamp

KW - Single cell RT-PCR

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