MRI of transgene expression: Correlation to therapeutic gene expression

Tomotsugu Ichikawa, Dagmar Högemann, Yoshinaga Saeki, Edyta Tyminski, Kinya Terada, Ralph Weissleder, E. Antonio Chiocca, James P. Basilion

Research output: Contribution to journalArticle

109 Citations (Scopus)

Abstract

Magnetic resonance imaging (MRI) can provide high-resolution 3D maps of structural and functional information, yet its use of mapping in vivo gene expression has only recently been explored. A potential application for this technology is to noninvasively image transgene expression. The current study explores the latter using a nonregulatable internalizing engineered transferrin receptor (ETR) whose expression can be probed for with a superparamagnetic Tf-CLIO probe. Using an HSV-based amplicon vector system for transgene delivery, we demonstrate that: 1) ETR is a sensitive MR marker gene; 2) several transgenes can be efficiently expressed from a single amplicon; 3) expression of each transgene results in functional gene product; and 4) ETR gene expression correlates with expression of therapeutic genes when the latter are contained within the same amplicon. These data, taken together, suggest that MRI of ETR expression can serve as a surrogate for measuring therapeutic transgene expression.

Original languageEnglish
Pages (from-to)523-530
Number of pages8
JournalNeoplasia (United States)
Volume4
Issue number6
DOIs
Publication statusPublished - Nov 2002
Externally publishedYes

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Keywords

  • Amplicon
  • CLIO
  • ETR
  • MRI
  • Transgene

ASJC Scopus subject areas

  • Cancer Research

Cite this

Ichikawa, T., Högemann, D., Saeki, Y., Tyminski, E., Terada, K., Weissleder, R., Chiocca, E. A., & Basilion, J. P. (2002). MRI of transgene expression: Correlation to therapeutic gene expression. Neoplasia (United States), 4(6), 523-530. https://doi.org/10.1038/sj.neo.7900266