MRI-guided neutron capture therapy by use of a dual gadolinium/boron agent targeted at tumour cells through upregulated low-density lipoprotein transporters

Simonetta Geninatti-Crich, Diego Alberti, Ibolya Szabo, Annamaria Deagostino, Antonio Toppino, Alessandro Barge, Francesca Ballarini, Silva Bortolussi, Piero Bruschi, Nicoletta Protti, Sabrina Stella, Saverio Altieri, Paolo Venturello, Silvio Aime

Research output: Contribution to journalArticlepeer-review

42 Citations (Scopus)

Abstract

The upregulation of low-density lipoprotein (LDL) transporters in tumour cells has been exploited to deliver a sufficient amount of gadolinium/boron/ ligand (Gd/B/L) probes for neutron capture therapy, a binary chemio-radiotherapy for cancer treatment. The Gd/B/L probe consists of a carborane unit (ten B atoms) bearing an aliphatic chain on one side (to bind LDL particles), and a GdIII/1,4,7,10-tetraazacyclododecane monoamide complex on the other (for detection by magnetic resonance imaging (MRI)). Up to 190Gd/B/L probes were loaded per LDL particle. The uptake from tumour cells was initially assessed on cell cultures of human hepatoma (HepG2), murine melanoma (B16), and human glioblastoma (U87). The MRI assessment of the amount of Gd/B/L taken up by tumour cells was validated by inductively coupled plasma-mass-spectrometric measurements of the Gd and B content. Measurements were undertaken in vivo on mice bearing tumours in which B16 tumour cells were inoculated at the base of the neck. From the acquisition of magnetic resonance images, it was established that after 4-6hours from the administration of the Gd/B/L-LDL particles (0.1 and 1mmol kg-1 of Gd and 10B, respectively) the amount of boron taken up in the tumour region is above the threshold required for successful NCT treatment. After neutron irradiation, tumour growth was followed for 20days by MRI. The group of treated mice showed markedly lower tumour growth with respect to the control group.

Original languageEnglish
Pages (from-to)8479-8486
Number of pages8
JournalChemistry - A European Journal
Volume17
Issue number30
DOIs
Publication statusPublished - Jul 18 2011
Externally publishedYes

Keywords

  • boron
  • gadolinium
  • lipoproteins
  • magnetic resonance imaging
  • nanotechnology
  • neutron capture therapy

ASJC Scopus subject areas

  • Catalysis
  • Organic Chemistry

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