MPK9 and MPK12 function in SA-induced stomatal closure in arabidopsis thaliana

Md Atiqur Rahman Khokon, Mohammad Abdus Salam, Fabien Jammes, Wenxiu Ye, Mohammad Anowar Hossain, Eiji Okuma, Yoshimasa Nakamura, Izumi Mori, June M. Kwak, Yoshiyuki Murata

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

Salicylic acid (SA) induces stomatal closure sharing several components with abscisic acid (ABA) and methyl jasmonate (MeJA) signaling. We have previously shown that two guard cell-preferential mitogen-activated protein kinases (MAPKs), MPK9 and MPK12, positively regulate ABA signaling and MeJA signaling in Arabidopsis thaliana. In this study, we examined whether these two MAPKs are involved in SA-induced stomatal closure using genetic mutants and a pharmacological, MAPKK inhibitor. Salicylic acid induced stomatal closure in mpk9 and mpk12 single mutants but not in mpk9 mpk12 double mutants. The MAPKK inhibitor PD98059 inhibited SA-induced stomatal closure in wild-type plants. Salicylic acid induced extracellular reactive oxygen species (ROS) production, intracellular ROS accumulation, and cytosolic alkalization in the mpk9, mpk12, and mpk9 mpk12 mutants. Moreover, SA-activated S-type anion channels in guard cells of wild-type plants but not in guard cells of mpk9 mpk12 double mutants. These results imply that MPK9 and MPK12 are positive regulators of SA signaling in Arabidopsis guard cells.

Original languageEnglish
Pages (from-to)1394-1400
Number of pages7
JournalBioscience, Biotechnology and Biochemistry
Volume81
Issue number7
DOIs
Publication statusPublished - 2017

Fingerprint

Salicylic Acid
Arabidopsis
Abscisic Acid
Mitogen-Activated Protein Kinase Kinases
Mitogen-Activated Protein Kinases
Reactive Oxygen Species
Plant Cells
Anions
Pharmacology

Keywords

  • Cytosolic alkalization
  • Mitogen-activated protein kinase
  • Reactive oxygen species
  • S-type anion channel in guard cells
  • Salicylic acid

ASJC Scopus subject areas

  • Analytical Chemistry
  • Biotechnology
  • Biochemistry
  • Applied Microbiology and Biotechnology
  • Molecular Biology
  • Organic Chemistry

Cite this

MPK9 and MPK12 function in SA-induced stomatal closure in arabidopsis thaliana. / Khokon, Md Atiqur Rahman; Salam, Mohammad Abdus; Jammes, Fabien; Ye, Wenxiu; Hossain, Mohammad Anowar; Okuma, Eiji; Nakamura, Yoshimasa; Mori, Izumi; Kwak, June M.; Murata, Yoshiyuki.

In: Bioscience, Biotechnology and Biochemistry, Vol. 81, No. 7, 2017, p. 1394-1400.

Research output: Contribution to journalArticle

Khokon, Md Atiqur Rahman ; Salam, Mohammad Abdus ; Jammes, Fabien ; Ye, Wenxiu ; Hossain, Mohammad Anowar ; Okuma, Eiji ; Nakamura, Yoshimasa ; Mori, Izumi ; Kwak, June M. ; Murata, Yoshiyuki. / MPK9 and MPK12 function in SA-induced stomatal closure in arabidopsis thaliana. In: Bioscience, Biotechnology and Biochemistry. 2017 ; Vol. 81, No. 7. pp. 1394-1400.
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