Mouse ten-m/odz is a new family of dimeric type II transmembrane proteins expressed in many tissues

Toshitaka Oohashi, Xiao Hong Zhou, Kang Feng, Brigitta Richter, Matthias Mörgelin, Maria Thereza Perez, Wei Dong Su, Ruth Chiquet-Ehrismann, Uwe Rauch, Reinhard Fässler

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81 Citations (Scopus)

Abstract

The Drosophila gene ten-m/odz is the only pair rule gene identified to date which is not a transcription factor. In an attempt to analyze the structure and the function of ten-m/odz in mouse, we isolated four murine ten-m cDNAs which code for proteins of 2,700-2,800 amino acids. All four proteins (Ten-m1-4) lack signal peptides at the NH2 terminus, but contain a short hydrophobic domain characteristic of transmembrane proteins, 300-400 amino acids after the NH2 terminus. About 200 amino acids COOH-terminal to this hydrophobic region are eight consecutive EGF-like domains. Cell transfection, biochemical, and electronmicroscopic studies suggest that Ten- m1 is a dimeric type II transmembrane protein. Expression of fusion proteins composed of the NH2-terminal and hydrophobic domain of ten-m1 attached to the alkaline phosphatase reporter gene resulted in membrane-associated staining of the alkaline phosphatase. Electronmicroscopic and electrophoretic analysis of a secreted form of the extracellular domain of Ten-m1 showed that Ten-m1 is a disulfide-linked dimer and that the dimerization is mediated by EGF-like modules 2 and 5 which contain an odd number of cysteines. Northern blot and immunohistochemical analyses revealed widespread expression of mouse ten-m genes, with most prominent expression in brain. All four ten-m genes can be expressed in variously spliced mRNA isoforms. The extracellular domain of Ten-m1 fused to an alkaline phosphatase reporter bound to specific regions in many tissues which were partially overlapping with the Ten-m1 immunostaining. Far Western assays and electronmicroscopy demonstrated that Ten-m1 can bind to itself.

Original languageEnglish
Pages (from-to)563-577
Number of pages15
JournalJournal of Cell Biology
Volume145
Issue number3
DOIs
Publication statusPublished - May 3 1999

Keywords

  • Epidermal growth factor
  • Pair rule
  • Ten-m/odz
  • Transmembrane protein

ASJC Scopus subject areas

  • Cell Biology

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    Oohashi, T., Zhou, X. H., Feng, K., Richter, B., Mörgelin, M., Perez, M. T., Su, W. D., Chiquet-Ehrismann, R., Rauch, U., & Fässler, R. (1999). Mouse ten-m/odz is a new family of dimeric type II transmembrane proteins expressed in many tissues. Journal of Cell Biology, 145(3), 563-577. https://doi.org/10.1083/jcb.145.3.563