TY - JOUR
T1 - Mouse hepatic microsomal oxidation of aliphatic aldehydes (C8 to C011) to carboxylic acids
AU - Watanabe, Kazuhito
AU - Matsunaga, Tamihide
AU - Narimatsu, Shizuo
AU - Yamamoto, Ikuo
AU - Yoshimura, Hiddetoshi
N1 - Funding Information:
We thank Miss A. Nakashima of Analytical Center of llokuriku University for carrying out Cc-m analyses. lhis research was supported in part by a Grant-in-Aid from Scientific Research provided by the Ministry of Education, Science and Culture of Japan.
PY - 1992/10/15
Y1 - 1992/10/15
N2 - Addition of saturated and α, β-unsaturated aliphatic aldehydes (C8 to C11) significantly increased NADPH oxidation with mouse hepatic microsomes, and the aldehydes themselves were oxidized to the corresponding carboxylic acids. When these aldehyde substrates were incubated similarly under oxygen-18 gas and the carboxylic acids formed were analyzed by GC-MS after methylation, it was indicated that oxygen-18 was significantly incorporated into the carboxylic acids formed from α,β-unsaturated aldehydes, but not significantly into the carboxylic acids formed from saturated aldehydes. These results indicate that enzyme and/or mechanism responsible for the oxidation of these two types of aldehydes is different from each other.
AB - Addition of saturated and α, β-unsaturated aliphatic aldehydes (C8 to C11) significantly increased NADPH oxidation with mouse hepatic microsomes, and the aldehydes themselves were oxidized to the corresponding carboxylic acids. When these aldehyde substrates were incubated similarly under oxygen-18 gas and the carboxylic acids formed were analyzed by GC-MS after methylation, it was indicated that oxygen-18 was significantly incorporated into the carboxylic acids formed from α,β-unsaturated aldehydes, but not significantly into the carboxylic acids formed from saturated aldehydes. These results indicate that enzyme and/or mechanism responsible for the oxidation of these two types of aldehydes is different from each other.
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U2 - 10.1016/0006-291X(92)92357-4
DO - 10.1016/0006-291X(92)92357-4
M3 - Article
C2 - 1417834
AN - SCOPUS:0026468314
VL - 188
SP - 114
EP - 119
JO - Biochemical and Biophysical Research Communications
JF - Biochemical and Biophysical Research Communications
SN - 0006-291X
IS - 1
ER -