Mouse embryo motion and embryonic development from the 2-cell to blastocyst stage using mechanical vibration systems

Yuka Asano, Koji Matsuura

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

We investigated the effect of mechanical stimuli on mouse embryonic development from the 2-cell to blastocyst stage to evaluate physical factors affecting embryonic development. Shear stress (SS) applied to embryos using two mechanical vibration systems (MVSs) was calculated by observing microscopic images of moving embryos during mechanical vibration (MV). The MVSs did not induce any motion of the medium and the diffusion rate using MVSs was the same as that under static conditions. Three days of culture using MVS did not improve embryonic development. MVS transmitted MV power more efficiently to embryos than other systems and resulted in a significant decrease in development to the morula or blastocyst stage after 2 days. Comparison of the results of embryo culture using dynamic culture systems demonstrated that macroscopic diffusion of secreted materials contributes to improved development of mouse embryos to the blastocyst stage. These results also suggest that the threshold of SS and MV to induce negative effects for mouse embryos at stages earlier than the blastocyst may be lower than that for the blastocyst, and that mouse embryos are more sensitive to physical and chemical stimuli than human or pig embryos because of their thinner zona pellucida.

Original languageEnglish
Pages (from-to)733-741
Number of pages9
JournalReproduction, Fertility and Development
Volume26
Issue number5
DOIs
Publication statusPublished - 2014

Fingerprint

Blastocyst
Vibration
vibration
blastocyst
Embryonic Development
embryo (animal)
embryogenesis
Embryonic Structures
mice
cells
shear stress
Morula
Zona Pellucida
Mechanical Stress
zona pellucida
embryo culture
morula
Swine
swine

Keywords

  • Dynamic culture system
  • Mechanical stimuli
  • Shear stress

ASJC Scopus subject areas

  • Animal Science and Zoology
  • Reproductive Medicine
  • Developmental Biology
  • Endocrinology
  • Genetics
  • Molecular Biology
  • Biotechnology
  • Medicine(all)

Cite this

Mouse embryo motion and embryonic development from the 2-cell to blastocyst stage using mechanical vibration systems. / Asano, Yuka; Matsuura, Koji.

In: Reproduction, Fertility and Development, Vol. 26, No. 5, 2014, p. 733-741.

Research output: Contribution to journalArticle

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