Motility-mediated regulation of virulence in Pseudomonas syringae

Yuki Ichinose; Takahiro Sawada; Hidenori Matsui; Mikihiro Yamamoto; Kazuhiro Toyoda; Yoshiteru Noutoshi; Fumiko Taguchi

doi:10.1016/j.pmpp.2016.02.005

Motility-mediated regulation of virulence in Pseudomonas syringae

Yuki Ichinose, Takahiro Sawada, Hidenori Matsui, Mikihiro Yamamoto, Kazuhiro Toyoda, Yoshiteru Noutoshi, Fumiko Taguchi

Research output: Contribution to journal › Article

1 Citation (Scopus)

Abstract

We have investigated Pseudomonas syringae pv. tabaci-plant interactions using a large variety of virulence-related mutants. A flagellin-defective mutant, δ. fliC, lost flagellar motility and the ability to produce N-acyl homoserine lactones; it had reduced ability to cause disease symptoms, but the expression of genes encoding a multidrug efflux pump transporter, mexEFoprN, was activated. A type IV pili (T4P)-defective mutant, δ. pilA, lost swarming motility, had reduced expression of hrp-related genes and virulence toward the host tobacco plant, but expression of the genes encoding another multidrug efflux pump transporter, mexABoprM, was activated. These results suggest that the genes regulating flagella- and T4P-mediated motilities also regulate expression of other virulence-related genes.

Original language	English
Journal	Physiological and Molecular Plant Pathology
DOIs	https://doi.org/10.1016/j.pmpp.2016.02.005
Publication status	Accepted/In press - Dec 8 2015

Keywords

Flagellar motility
Multidrug efflux pump transporter
Quorum sensing
Type III secretion system
Type IV pili-mediated motility

ASJC Scopus subject areas

Plant Science
Genetics

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Ichinose, Y., Sawada, T., Matsui, H., Yamamoto, M., Toyoda, K., Noutoshi, Y., & Taguchi, F. (Accepted/In press). Motility-mediated regulation of virulence in Pseudomonas syringae. Physiological and Molecular Plant Pathology. https://doi.org/10.1016/j.pmpp.2016.02.005

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title = "Motility-mediated regulation of virulence in Pseudomonas syringae",

abstract = "We have investigated Pseudomonas syringae pv. tabaci-plant interactions using a large variety of virulence-related mutants. A flagellin-defective mutant, δ. fliC, lost flagellar motility and the ability to produce N-acyl homoserine lactones; it had reduced ability to cause disease symptoms, but the expression of genes encoding a multidrug efflux pump transporter, mexEFoprN, was activated. A type IV pili (T4P)-defective mutant, δ. pilA, lost swarming motility, had reduced expression of hrp-related genes and virulence toward the host tobacco plant, but expression of the genes encoding another multidrug efflux pump transporter, mexABoprM, was activated. These results suggest that the genes regulating flagella- and T4P-mediated motilities also regulate expression of other virulence-related genes.",

keywords = "Flagellar motility, Multidrug efflux pump transporter, Quorum sensing, Type III secretion system, Type IV pili-mediated motility",

author = "Yuki Ichinose and Takahiro Sawada and Hidenori Matsui and Mikihiro Yamamoto and Kazuhiro Toyoda and Yoshiteru Noutoshi and Fumiko Taguchi",

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AU - Ichinose, Yuki

AU - Sawada, Takahiro

AU - Matsui, Hidenori

AU - Yamamoto, Mikihiro

AU - Toyoda, Kazuhiro

AU - Noutoshi, Yoshiteru

AU - Taguchi, Fumiko

PY - 2015/12/8

Y1 - 2015/12/8

N2 - We have investigated Pseudomonas syringae pv. tabaci-plant interactions using a large variety of virulence-related mutants. A flagellin-defective mutant, δ. fliC, lost flagellar motility and the ability to produce N-acyl homoserine lactones; it had reduced ability to cause disease symptoms, but the expression of genes encoding a multidrug efflux pump transporter, mexEFoprN, was activated. A type IV pili (T4P)-defective mutant, δ. pilA, lost swarming motility, had reduced expression of hrp-related genes and virulence toward the host tobacco plant, but expression of the genes encoding another multidrug efflux pump transporter, mexABoprM, was activated. These results suggest that the genes regulating flagella- and T4P-mediated motilities also regulate expression of other virulence-related genes.

AB - We have investigated Pseudomonas syringae pv. tabaci-plant interactions using a large variety of virulence-related mutants. A flagellin-defective mutant, δ. fliC, lost flagellar motility and the ability to produce N-acyl homoserine lactones; it had reduced ability to cause disease symptoms, but the expression of genes encoding a multidrug efflux pump transporter, mexEFoprN, was activated. A type IV pili (T4P)-defective mutant, δ. pilA, lost swarming motility, had reduced expression of hrp-related genes and virulence toward the host tobacco plant, but expression of the genes encoding another multidrug efflux pump transporter, mexABoprM, was activated. These results suggest that the genes regulating flagella- and T4P-mediated motilities also regulate expression of other virulence-related genes.

KW - Flagellar motility

KW - Multidrug efflux pump transporter

KW - Quorum sensing

KW - Type III secretion system

KW - Type IV pili-mediated motility

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