Sequential changes in the development of ethylnitrosourea (ENU)‐induced rat brain tumors were examined histologically, immunohistochemically, electron microscopically and autoradiographically. In 47 Sprague‐Dawley rats transplacentally administrated with ENU, 95 brain tumors developed, including 76 microtumors less than lmm in diameter. Microtumors were found mainly in the paraventricular area, but some were found in the peripheral brain tissue. They were composed of small tumor cells which had round dark nuclei and scanty cytoplasm immunohistochemically negative for Leu 7 and glial Abrillary acidic protein (CFAP). The 19 macrotumors were mature gliomas, 3 of which histologically corresponded to oligodendrogliomas and 16 to mixed gliomas. The tumor cells of the former had small round nuclei with distinct perinuclear halos and a small amount of cytoplasm positive for Leu 7. The latter were chiefly composed of polygonal cells having large round nuclei and rich cytoplasm positive for CFAP. An autoradiographic study using 3H‐thymidine revealed that the labeling index of the tumor cells was high in mixed gliomas and microtumors, but low in oligodendrogliomas. It may be concluded that the constituent cells of microtumors correspond to glioblasts or migrating neuroglias, which gradually mature to form oligodendrogliomas or astrocytomas. ACTA PATHOL. JPN. 35 : 1385–1396, 1985.
|Number of pages||12|
|Publication status||Published - Nov 1985|
ASJC Scopus subject areas
- Pathology and Forensic Medicine