Morphological interactions of interdigitating dendritic cells with B and T cells in human mesenteric lymph nodes

Kiyoshi Takahashi, Asagoe Kenji, Teramoto Norihiro, Kondo Eisaku, Takashi Oka, Hayashi Kazuhiko, Tadashi Yoshino, Akagi Tadaatsu

Research output: Contribution to journalArticle

22 Citations (Scopus)

Abstract

Interdigitating dendritic cells (IDC) of the human mesenteric lymph nodes (LN) were examined by two-color immunofluorescent microscopy and flow cytometry to clarify their exact localization, immunophenotype, and relationships with T and B cells. IDC were identified as HLA-DRbright large dendriform cells of the T cell areas co-expressing CD40, CD54 (ICAM-1), CD80 (B7/B7-1), CD83, and CD86 (B70/B7-2). The majority of EDC directly attached to a few IgD+ naive B cells as well as to numerous CD4+ T cells. When LN cells were singly suspended and briefly incubated in vitro, IDC formed clusters with IgD+ IgM+ naive B cells, but not with IgA+ or IgG+ B cells. When suspended LN cells were cultured, clustered B cells disappeared within 7 days, and on prolonged culture, some IDC developed into extensively dendriform cells forming stable complexes with several or sometimes numerous CD4+ IL-2R+ CD40L+ activated T cells. These findings indicate that resting naive B cells actually interact with BDC directly in T cell areas of human secondary lymphoid tissues. IDC have a non-antigen (Ag)-specific, strong affinity for resting naive B cells, but this affinity is transient and IDC cannot form stable complexes with B cells, although they can form stable complexes with activated T cells. It is suggested that the stable IDC/Ag-activated T cell complexes make it possible to capture and to stimulate rare Ag-specific resting naive B cells with high efficiency.

Original languageEnglish
Pages (from-to)131-138
Number of pages8
JournalAmerican Journal of Pathology
Volume159
Issue number1
Publication statusPublished - Jul 2001

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Dendritic Cells
B-Lymphocytes
Lymph Nodes
T-Lymphocytes
Immunoglobulin D
Antigens
CD40 Ligand
Lymphoid Tissue
Intercellular Adhesion Molecule-1
Immunoglobulin A
Immunoglobulin M
Microscopy
Cultured Cells
Flow Cytometry
Color
Immunoglobulin G

ASJC Scopus subject areas

  • Pathology and Forensic Medicine

Cite this

Takahashi, K., Kenji, A., Norihiro, T., Eisaku, K., Oka, T., Kazuhiko, H., ... Tadaatsu, A. (2001). Morphological interactions of interdigitating dendritic cells with B and T cells in human mesenteric lymph nodes. American Journal of Pathology, 159(1), 131-138.

Morphological interactions of interdigitating dendritic cells with B and T cells in human mesenteric lymph nodes. / Takahashi, Kiyoshi; Kenji, Asagoe; Norihiro, Teramoto; Eisaku, Kondo; Oka, Takashi; Kazuhiko, Hayashi; Yoshino, Tadashi; Tadaatsu, Akagi.

In: American Journal of Pathology, Vol. 159, No. 1, 07.2001, p. 131-138.

Research output: Contribution to journalArticle

Takahashi, K, Kenji, A, Norihiro, T, Eisaku, K, Oka, T, Kazuhiko, H, Yoshino, T & Tadaatsu, A 2001, 'Morphological interactions of interdigitating dendritic cells with B and T cells in human mesenteric lymph nodes', American Journal of Pathology, vol. 159, no. 1, pp. 131-138.
Takahashi, Kiyoshi ; Kenji, Asagoe ; Norihiro, Teramoto ; Eisaku, Kondo ; Oka, Takashi ; Kazuhiko, Hayashi ; Yoshino, Tadashi ; Tadaatsu, Akagi. / Morphological interactions of interdigitating dendritic cells with B and T cells in human mesenteric lymph nodes. In: American Journal of Pathology. 2001 ; Vol. 159, No. 1. pp. 131-138.
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