Abstract
We characterized the morphological expression of glomerular endothelial cells (GEC) cultured on a basement membrane matrix, Matrigel. GEC cultured on this matrix formed abundant networks of branching and anastomosing cords of cells, and formed capillary-like structures with a lumen and interdigitating junctional processes. In contrast, GEC cultured on fibronectin-coated dishes had elongated spindle shapes without any interdigitating junctional processes. These findings suggest that the culture system with Matrigel is useful for inducing and maintaining characteristics close to in vivo GEC.
| Original language | English |
|---|---|
| Pages (from-to) | 1-8 |
| Number of pages | 8 |
| Journal | the japanese journal of nephrology |
| Volume | 36 |
| Issue number | 1 |
| DOIs | |
| Publication status | Published - Jan 1 1994 |
| Externally published | Yes |
Keywords
- Matrigel
- angiogenesis
- cultured glomerular endothelial cells
- morphological study
- tube-like structures
ASJC Scopus subject areas
- Nephrology
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Tsuchida, S., Hiraoka, M., Tsukahara, H., Negami, A. I., Tominaga, T., & Sudo, M. (1994). Morphological characterization of glomerular endothelial cells cultured on a basement membrane matrix. the japanese journal of nephrology, 36(1), 1-8. https://doi.org/10.14842/jpnjnephrol1959.36.1