Morphological characterization of glomerular endothelial cells cultured on a basement membrane matrix

Shinya Tsuchida, Masahiro Hiraoka, Hirokazu Tsukahara, Akira I. Negami, Toshiro Tominaga, Masakatsu Sudo

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

We characterized the morphological expression of glomerular endothelial cells (GEC) cultured on a basement membrane matrix, Matrigel. GEC cultured on this matrix formed abundant networks of branching and anastomosing cords of cells, and formed capillary-like structures with a lumen and interdigitating junctional processes. In contrast, GEC cultured on fibronectin-coated dishes had elongated spindle shapes without any interdigitating junctional processes. These findings suggest that the culture system with Matrigel is useful for inducing and maintaining characteristics close to in vivo GEC.

Original languageEnglish
Pages (from-to)1-8
Number of pages8
Journalthe japanese journal of nephrology
Volume36
Issue number1
DOIs
Publication statusPublished - Jan 1 1994
Externally publishedYes

Keywords

  • Matrigel
  • angiogenesis
  • cultured glomerular endothelial cells
  • morphological study
  • tube-like structures

ASJC Scopus subject areas

  • Nephrology

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