Abstract
We characterized the morphological expression of glomerular endothelial cells (GEC) cultured on a basement membrane matrix, Matrigel. GEC cultured on this matrix formed abundant networks of branching and anastomosing cords of cells, and formed capillary-like structures with a lumen and interdigitating junctional processes. In contrast, GEC cultured on fibronectin-coated dishes had elongated spindle shapes without any interdigitating junctional processes. These findings suggest that the culture system with Matrigel is useful for inducing and maintaining characteristics close to in vivo GEC.
| Original language | English |
|---|---|
| Pages (from-to) | 1-8 |
| Number of pages | 8 |
| Journal | Nippon Jinzo Gakkai shi |
| Volume | 36 |
| Issue number | 1 |
| Publication status | Published - Jan 1994 |
| Externally published | Yes |
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ASJC Scopus subject areas
- Nephrology
Cite this
Morphological characterization of glomerular endothelial cells cultured on a basement membrane matrix. / Tsuchida, S.; Hiraoka, M.; Tsukahara, Hirokazu; Negami, A. I.; Tominaga, T.; Sudo, M.
In: Nippon Jinzo Gakkai shi, Vol. 36, No. 1, 01.1994, p. 1-8.Research output: Contribution to journal › Article
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TY - JOUR
T1 - Morphological characterization of glomerular endothelial cells cultured on a basement membrane matrix.
AU - Tsuchida, S.
AU - Hiraoka, M.
AU - Tsukahara, Hirokazu
AU - Negami, A. I.
AU - Tominaga, T.
AU - Sudo, M.
PY - 1994/1
Y1 - 1994/1
N2 - We characterized the morphological expression of glomerular endothelial cells (GEC) cultured on a basement membrane matrix, Matrigel. GEC cultured on this matrix formed abundant networks of branching and anastomosing cords of cells, and formed capillary-like structures with a lumen and interdigitating junctional processes. In contrast, GEC cultured on fibronectin-coated dishes had elongated spindle shapes without any interdigitating junctional processes. These findings suggest that the culture system with Matrigel is useful for inducing and maintaining characteristics close to in vivo GEC.
AB - We characterized the morphological expression of glomerular endothelial cells (GEC) cultured on a basement membrane matrix, Matrigel. GEC cultured on this matrix formed abundant networks of branching and anastomosing cords of cells, and formed capillary-like structures with a lumen and interdigitating junctional processes. In contrast, GEC cultured on fibronectin-coated dishes had elongated spindle shapes without any interdigitating junctional processes. These findings suggest that the culture system with Matrigel is useful for inducing and maintaining characteristics close to in vivo GEC.
UR - http://www.scopus.com/inward/record.url?scp=0028017230&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0028017230&partnerID=8YFLogxK
M3 - Article
C2 - 8107303
AN - SCOPUS:0028017230
VL - 36
SP - 1
EP - 8
JO - Japanese Journal of Nephrology
JF - Japanese Journal of Nephrology
SN - 0385-2385
IS - 1
ER -