Morphological characterization of glomerular endothelial cells cultured on a basement membrane matrix.

S. Tsuchida, M. Hiraoka, Hirokazu Tsukahara, A. I. Negami, T. Tominaga, M. Sudo

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

We characterized the morphological expression of glomerular endothelial cells (GEC) cultured on a basement membrane matrix, Matrigel. GEC cultured on this matrix formed abundant networks of branching and anastomosing cords of cells, and formed capillary-like structures with a lumen and interdigitating junctional processes. In contrast, GEC cultured on fibronectin-coated dishes had elongated spindle shapes without any interdigitating junctional processes. These findings suggest that the culture system with Matrigel is useful for inducing and maintaining characteristics close to in vivo GEC.

Original languageEnglish
Pages (from-to)1-8
Number of pages8
JournalNippon Jinzo Gakkai shi
Volume36
Issue number1
Publication statusPublished - Jan 1994
Externally publishedYes

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Basement Membrane
Endothelial Cells
Fibronectins
matrigel

ASJC Scopus subject areas

  • Nephrology

Cite this

Morphological characterization of glomerular endothelial cells cultured on a basement membrane matrix. / Tsuchida, S.; Hiraoka, M.; Tsukahara, Hirokazu; Negami, A. I.; Tominaga, T.; Sudo, M.

In: Nippon Jinzo Gakkai shi, Vol. 36, No. 1, 01.1994, p. 1-8.

Research output: Contribution to journalArticle

Tsuchida, S, Hiraoka, M, Tsukahara, H, Negami, AI, Tominaga, T & Sudo, M 1994, 'Morphological characterization of glomerular endothelial cells cultured on a basement membrane matrix.', Nippon Jinzo Gakkai shi, vol. 36, no. 1, pp. 1-8.
Tsuchida, S. ; Hiraoka, M. ; Tsukahara, Hirokazu ; Negami, A. I. ; Tominaga, T. ; Sudo, M. / Morphological characterization of glomerular endothelial cells cultured on a basement membrane matrix. In: Nippon Jinzo Gakkai shi. 1994 ; Vol. 36, No. 1. pp. 1-8.
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