Morphological characterization of glomerular endothelial cells cultured on a basement membrane matrix

Shinya Tsuchida; Masahiro Hiraoka; Hirokazu Tsukahara; Akira I. Negami; Toshiro Tominaga; Masakatsu Sudo

doi:10.14842/jpnjnephrol1959.36.1

Morphological characterization of glomerular endothelial cells cultured on a basement membrane matrix

Shinya Tsuchida, Masahiro Hiraoka, Hirokazu Tsukahara, Akira I. Negami, Toshiro Tominaga, Masakatsu Sudo

Research output: Contribution to journal › Article › peer-review

1 Citation (Scopus)

Abstract

We characterized the morphological expression of glomerular endothelial cells (GEC) cultured on a basement membrane matrix, Matrigel. GEC cultured on this matrix formed abundant networks of branching and anastomosing cords of cells, and formed capillary-like structures with a lumen and interdigitating junctional processes. In contrast, GEC cultured on fibronectin-coated dishes had elongated spindle shapes without any interdigitating junctional processes. These findings suggest that the culture system with Matrigel is useful for inducing and maintaining characteristics close to in vivo GEC.

Original language	English
Pages (from-to)	1-8
Number of pages	8
Journal	the japanese journal of nephrology
Volume	36
Issue number	1
DOIs	https://doi.org/10.14842/jpnjnephrol1959.36.1
Publication status	Published - 1994
Externally published	Yes

Keywords

Matrigel
angiogenesis
cultured glomerular endothelial cells
morphological study
tube-like structures

ASJC Scopus subject areas

Nephrology

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abstract = "We characterized the morphological expression of glomerular endothelial cells (GEC) cultured on a basement membrane matrix, Matrigel. GEC cultured on this matrix formed abundant networks of branching and anastomosing cords of cells, and formed capillary-like structures with a lumen and interdigitating junctional processes. In contrast, GEC cultured on fibronectin-coated dishes had elongated spindle shapes without any interdigitating junctional processes. These findings suggest that the culture system with Matrigel is useful for inducing and maintaining characteristics close to in vivo GEC.",

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AU - Tsuchida, Shinya

AU - Hiraoka, Masahiro

AU - Tsukahara, Hirokazu

AU - Negami, Akira I.

AU - Tominaga, Toshiro

AU - Sudo, Masakatsu

PY - 1994

Y1 - 1994

N2 - We characterized the morphological expression of glomerular endothelial cells (GEC) cultured on a basement membrane matrix, Matrigel. GEC cultured on this matrix formed abundant networks of branching and anastomosing cords of cells, and formed capillary-like structures with a lumen and interdigitating junctional processes. In contrast, GEC cultured on fibronectin-coated dishes had elongated spindle shapes without any interdigitating junctional processes. These findings suggest that the culture system with Matrigel is useful for inducing and maintaining characteristics close to in vivo GEC.

AB - We characterized the morphological expression of glomerular endothelial cells (GEC) cultured on a basement membrane matrix, Matrigel. GEC cultured on this matrix formed abundant networks of branching and anastomosing cords of cells, and formed capillary-like structures with a lumen and interdigitating junctional processes. In contrast, GEC cultured on fibronectin-coated dishes had elongated spindle shapes without any interdigitating junctional processes. These findings suggest that the culture system with Matrigel is useful for inducing and maintaining characteristics close to in vivo GEC.

KW - Matrigel

KW - angiogenesis

KW - cultured glomerular endothelial cells

KW - morphological study

KW - tube-like structures

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