Morphological characterization of a new human epithelioid sarcoma cell line, ES020488, in vitro and in vivo

Hiroshi Sonobe, Mutsuo Furihata, Jun Iwata, Takashi Oka, Yuji Ohtsuki, Shinji Hamasato, Shigeyoshi Fujimoto

Research output: Contribution to journalArticle

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Abstract

A new human epithelioid sarcoma cell line (ES020488) was established from a cutaneous metastasis in 26-year-old man, and was morphologically characterized in vitro and in vivo by comparison with the original tumor. The ES020488 cells showed a male karyotype ranging from 39 to 83 chromosomes, with various abnormalities but no specific pattern. The cells were round, polygonal or spindle-shaped with abundant cytoplasm and round nuclei containing prominent nucleoli; they proliferated in a sheet-like pattern. Tumors transplanted into nude mice revealed essentially the same features as the original tumor. Both in vitro and in vivo, the cells immunohistochemically expressed vimentin, cytokeratin, and EMA, but not desmin and S-100 protein. Ultrastructural study revealed irregular or round nuclei containing abundant euchromatin and prominent nucleoli, many intermediate filaments running irregularly or around the nucleus, and a number of filopodia-like processes. ES020488 cells were thus proven to retain and exhibit the unique morphological characteristics of an epithelioid sarcoma both in vitro and in vivo. These cells are possibly derived from synovioblastic mesenchyme.

Original languageEnglish
Pages (from-to)219-225
Number of pages7
JournalVirchows Archiv B Cell Pathology Including Molecular Pathology
Volume63
Issue number1
DOIs
Publication statusPublished - Dec 1993
Externally publishedYes

Fingerprint

Epithelioid Cells
Sarcoma
Cell Line
Euchromatin
Neoplasms
Pseudopodia
Desmin
Intermediate Filaments
S100 Proteins
Vimentin
Mesoderm
Keratins
Karyotype
Nude Mice
Cytoplasm
Chromosomes
In Vitro Techniques
Neoplasm Metastasis
Skin

Keywords

  • Cell line
  • Characterization
  • Epithelioid sarcoma
  • Human

ASJC Scopus subject areas

  • Pathology and Forensic Medicine

Cite this

Morphological characterization of a new human epithelioid sarcoma cell line, ES020488, in vitro and in vivo. / Sonobe, Hiroshi; Furihata, Mutsuo; Iwata, Jun; Oka, Takashi; Ohtsuki, Yuji; Hamasato, Shinji; Fujimoto, Shigeyoshi.

In: Virchows Archiv B Cell Pathology Including Molecular Pathology, Vol. 63, No. 1, 12.1993, p. 219-225.

Research output: Contribution to journalArticle

Sonobe, Hiroshi ; Furihata, Mutsuo ; Iwata, Jun ; Oka, Takashi ; Ohtsuki, Yuji ; Hamasato, Shinji ; Fujimoto, Shigeyoshi. / Morphological characterization of a new human epithelioid sarcoma cell line, ES020488, in vitro and in vivo. In: Virchows Archiv B Cell Pathology Including Molecular Pathology. 1993 ; Vol. 63, No. 1. pp. 219-225.
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