Morphological and molecular phylogenetic analysis of the high triglyceride-producing marine diatom, Fistulifera solaris sp. nov. (Bacillariophyceae)

Mitsufumi Matsumoto, Shigeki Mayama, Michiko Nemoto, Yorikane Fukuda, Masaki Muto, Tomoko Yoshino, Tadashi Matsunaga, Tsuyoshi Tanaka

Research output: Contribution to journalArticle

21 Citations (Scopus)

Abstract

Summary: Fistulifera sp. strain JPCC DA0580, a marine pennate diatom, contains extremely high levels of intracellular triglyceride and has been suggested as a promising source of feedstock for biodiesel fuels. JPCC DA0580 was isolated from a mangrove swamp located in Sumiyo Bay below the mouths of the Sumiyo and Yakugachi Rivers in Amami-Ohshima, Kagoshima, Japan. In this study, the morphology and the 18S rDNA sequence of JPCC DA0580 were compared with those of other Fistulifera strains. JPCC DA0580 possesses morphological characters of the genus Fistulifera, namely lightly silicified frustules, a distinct median costa (raphe sternum), and a wart-like central pore (fistula). Morphometric analysis revealed that JPCC DA0580 differs from other Fistulifera species by the presence of a valve with coarser striation and coarser areolation. On the basis of 18S rDNA phylogeny, JPCC DA0580 formed a well-supported clade with other members of the Fistulifera species complex, although the number of nucleotide substitutions was highest in JPCC DA0580. Our results led us to propose the taxonomic name Fistulifera solaris sp. nov. for JPCC DA0580.

Original languageEnglish
Pages (from-to)257-268
Number of pages12
JournalPhycological Research
Volume62
Issue number4
DOIs
Publication statusPublished - Oct 1 2014
Externally publishedYes

Fingerprint

Diatoms
striation
Bacillariophyceae
species complex
Ribosomal DNA
mangrove
phylogeny
Triglycerides
substitution
diatom
triacylglycerols
mangrove swamps
phylogenetics
warts
sternum
Sternum
Warts
Biofuels
Wetlands
new species

Keywords

  • 18S rDNA
  • JPCC DA0580
  • Marine diatom
  • Morphometric analyses

ASJC Scopus subject areas

  • Ecology, Evolution, Behavior and Systematics
  • Physiology

Cite this

Morphological and molecular phylogenetic analysis of the high triglyceride-producing marine diatom, Fistulifera solaris sp. nov. (Bacillariophyceae). / Matsumoto, Mitsufumi; Mayama, Shigeki; Nemoto, Michiko; Fukuda, Yorikane; Muto, Masaki; Yoshino, Tomoko; Matsunaga, Tadashi; Tanaka, Tsuyoshi.

In: Phycological Research, Vol. 62, No. 4, 01.10.2014, p. 257-268.

Research output: Contribution to journalArticle

Matsumoto, Mitsufumi ; Mayama, Shigeki ; Nemoto, Michiko ; Fukuda, Yorikane ; Muto, Masaki ; Yoshino, Tomoko ; Matsunaga, Tadashi ; Tanaka, Tsuyoshi. / Morphological and molecular phylogenetic analysis of the high triglyceride-producing marine diatom, Fistulifera solaris sp. nov. (Bacillariophyceae). In: Phycological Research. 2014 ; Vol. 62, No. 4. pp. 257-268.
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