@article{56d7526cda5f412f8959a5ace3989242,
title = "Morphological and molecular identification of eimeria spp. In breeding chicken farms of Japan",
abstract = "There have been no reports of the prevalence of Eimeria spp. in poultry breeding farms in Japan unlike those of broiler farms. From 2017 to 2018, we examined the prevalence of Eimeria spp. on breeding farms in Japan by oocyst morphology and PCR analyses. A total of 143 samples was collected from 37 breeding farms in 21 prefectures of Japan. We detected oocysts of seven species at 34 of 37 breeding farms by PCR, and we identified E. brunetti at 51.5% of farms found to be positive for Eimeria. The differences in the identification of Eimeria spp. between the morphology and PCR assay methods of oocysts were pronounced for E. maxima and E. necatrix. We confirmed that molecular tools were more suitable for accurately estimating prevalence of Eimeria spp., and these findings suggest that E. brunetti could be widespread in Japan.",
keywords = "Breeding farm, Chicken, Eimeria, Eimeria brunetti, PCR",
author = "Makoto Matsubayashi and Tomoyuki Shibahara and Tomohide Matsuo and Toshimitsu Hatabu and Junya Yamagishi and Kazumi Sasai and Takashi Isobe",
note = "Funding Information: ACKNOWLEDGMENTS. The authors gratefully acknowledge the Research Institute for Animal Science in Biochemistry and Toxicology (Kanagawa, Japan) for conducting the fecal examinations and Mrs. Rika Sekiguchi and Mrs. Noriko Asama for help with the molecular identification assays. This work was supported in part by Grants-in-Aid from the Japan Poultry Breeders Hatcheries Association and the Joint Usage / Research Center (Research Center for Zoonosis Control, Hokkaido University). Funding Information: The authors gratefully acknowledge the Research Institute for Animal Science in Biochemistry and Toxicology (Kanagawa, Japan) for conducting the fecal examinations and Mrs. Rika Sekiguchi and Mrs. Noriko Asama for help with the molecular identification assays. This work was supported in part by Grants-in-Aid from the Japan Poultry Breeders Hatcheries Association and the Joint Usage / Research Center (Research Center for Zoonosis Control, Hokkaido University). Publisher Copyright: {\textcopyright} 2020 The Japanese Society of Veterinary Science.",
year = "2020",
doi = "10.1292/jvms.19-0661",
language = "English",
volume = "82",
pages = "516--519",
journal = "Journal of Veterinary Medical Science",
issn = "0916-7250",
publisher = "Japanese Society of Veterinary Science",
number = "5",
}