Morphological and molecular identification of eimeria spp. In breeding chicken farms of Japan

Makoto Matsubayashi, Tomoyuki Shibahara, Tomohide Matsuo, Toshimitsu Hatabu, Junya Yamagishi, Kazumi Sasai, Takashi Isobe

Research output: Contribution to journalComment/debatepeer-review

4 Citations (Scopus)


There have been no reports of the prevalence of Eimeria spp. in poultry breeding farms in Japan unlike those of broiler farms. From 2017 to 2018, we examined the prevalence of Eimeria spp. on breeding farms in Japan by oocyst morphology and PCR analyses. A total of 143 samples was collected from 37 breeding farms in 21 prefectures of Japan. We detected oocysts of seven species at 34 of 37 breeding farms by PCR, and we identified E. brunetti at 51.5% of farms found to be positive for Eimeria. The differences in the identification of Eimeria spp. between the morphology and PCR assay methods of oocysts were pronounced for E. maxima and E. necatrix. We confirmed that molecular tools were more suitable for accurately estimating prevalence of Eimeria spp., and these findings suggest that E. brunetti could be widespread in Japan.

Original languageEnglish
Pages (from-to)516-519
Number of pages4
JournalJournal of Veterinary Medical Science
Issue number5
Publication statusPublished - 2020


  • Breeding farm
  • Chicken
  • Eimeria
  • Eimeria brunetti
  • PCR

ASJC Scopus subject areas

  • veterinary(all)


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