Morphologic, flow cytometric, functional, and molecular analyses of S100B positive lymphocytes, unique cytotoxic lymphocytes containing S100B protein

Yukari Miki, Yuka Gion, Yuriko Mukae, Atsushi Hayashi, Hiaki Sato, Tadashi Yoshino, Kiyoshi Takahashi

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

Little is known about the S100B+ lymphocytes, which are unique human peripheral blood lymphocytes (PBL) containing the S100B protein. It has recently been shown that S100B is released from various types of S100B+ cells and exhibits varied cytokine-like activities. In this study, we precisely characterized the S100B+ lymphocytes of healthy adults with respect to the proportion in the whole PBL, immunophenotypes, function, and their S100B mRNA expression and also evaluated their S100B-releasing activity upon stimulation. S100B+ lymphocytes were detected in all individuals examined, and the proportion of S100B+ lymphocytes in the whole PBL ranged from 0.42% to 16.15% (mean, 4.21%). In addition, two subtypes of S100B+ lymphocytes, a CTL subtype (CD3+ CD8+ CD16-) and a NK subtype (CD3- CD8- CD16+), were detected. The majority of the CTL subtype of S100B+ lymphocytes expressed the αβ-T-cell receptor. Surprisingly, S100B mRNA was detected not only in S100B+ lymphocytes, but also in every S100B- lymphocytes, although the expression levels of S100B mRNA in S100B- lymphocytes were much lower than those of S100B+ lymphocytes. The CTL subtype of S100B+ lymphocytes exhibited blastic morphological changes, proliferated and released S100B upon stimulation with phytohemagglutinin. The NK subtype of S100B+ lymphocytes exhibited morphological NK activity when cocultivated with NK-sensitive target, K-562 cells. Thus, the CTL subtype of S100B+ lymphocytes exhibit the biological characteristics of T cells, while the NK subtype of S100B+ lymphocytes exhibit the characteristics of NK cells. These results suggest that S100B+ lymphocytes are a particular subtype of cytotoxic lymphocytes that play a unique role in antitumor immunity.

Original languageEnglish
Pages (from-to)99-110
Number of pages12
JournalEuropean Journal of Haematology
Volume90
Issue number2
DOIs
Publication statusPublished - Feb 2013

Fingerprint

Lymphocytes
Proteins
Messenger RNA
Phytohemagglutinins
T-Cell Antigen Receptor
Natural Killer Cells
Immunity
Cytokines

Keywords

  • Cytotoxic T cells
  • NK cells
  • Peripheral blood
  • S100B

ASJC Scopus subject areas

  • Hematology

Cite this

Morphologic, flow cytometric, functional, and molecular analyses of S100B positive lymphocytes, unique cytotoxic lymphocytes containing S100B protein. / Miki, Yukari; Gion, Yuka; Mukae, Yuriko; Hayashi, Atsushi; Sato, Hiaki; Yoshino, Tadashi; Takahashi, Kiyoshi.

In: European Journal of Haematology, Vol. 90, No. 2, 02.2013, p. 99-110.

Research output: Contribution to journalArticle

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