Morphologic degeneration of human microvascular endothelial cells induced by iodinated contrast media

W. Furuta, Toshiaki Sendo, Y. Kataoka, R. Oishi

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

Rationale and Objectives. The purpose of this study was to characterize the adverse effects of iohexol and ioxaglate on human microvascular endothelial cells, which may result in phlebitis, pain, and thrombosis. Materials and Methods. The degree of morphologic degeneration and of lactate dehydrogenase (LDH) efflux into the extracellular medium (as an index of cell viability) were determined in endothelial cell culture exposed for 10, 30, or 60 minutes to ioxaglate or iohexol (ionic and nonionic contrast media, respectively) at iodine concentrations of 100 or 150 mg/mL. Results. Ioxaglate induced concentration- and time-dependent morphologic degeneration, including shrinkage and loss of the cell tip in 20%-80% of endothelial cells; iohexol did not. After 60 minutes of exposure, ioxaglate at the higher concentration (150 mg iodine per milliliter) significantly increased the LDH signal (ie, the percentage of LDH released), to 20%. Conclusion. The present findings demonstrate that ioxaglate but not iohexol causes morphologic degeneration of the microvascular endothelial cells. This direct cytotoxic action of ioxaglate probably causes endothelial cell dysfunction, closely associated with the occurrence of phlebitis, pain, and thrombosis.

Original languageEnglish
Pages (from-to)158-161
Number of pages4
JournalAcademic Radiology
Volume8
Issue number2
Publication statusPublished - 2001
Externally publishedYes

Fingerprint

Ioxaglic Acid
Contrast Media
Iohexol
Endothelial Cells
L-Lactate Dehydrogenase
Phlebitis
Iodine
Thrombosis
Pain
Cell Survival
Cell Culture Techniques

Keywords

  • Contrast media
  • Effects
  • Experimental studies
  • Iohexol
  • Ioxaglate

ASJC Scopus subject areas

  • Radiology Nuclear Medicine and imaging

Cite this

Morphologic degeneration of human microvascular endothelial cells induced by iodinated contrast media. / Furuta, W.; Sendo, Toshiaki; Kataoka, Y.; Oishi, R.

In: Academic Radiology, Vol. 8, No. 2, 2001, p. 158-161.

Research output: Contribution to journalArticle

@article{23343cb8ba6f4022a1005faf24e2077f,
title = "Morphologic degeneration of human microvascular endothelial cells induced by iodinated contrast media",
abstract = "Rationale and Objectives. The purpose of this study was to characterize the adverse effects of iohexol and ioxaglate on human microvascular endothelial cells, which may result in phlebitis, pain, and thrombosis. Materials and Methods. The degree of morphologic degeneration and of lactate dehydrogenase (LDH) efflux into the extracellular medium (as an index of cell viability) were determined in endothelial cell culture exposed for 10, 30, or 60 minutes to ioxaglate or iohexol (ionic and nonionic contrast media, respectively) at iodine concentrations of 100 or 150 mg/mL. Results. Ioxaglate induced concentration- and time-dependent morphologic degeneration, including shrinkage and loss of the cell tip in 20{\%}-80{\%} of endothelial cells; iohexol did not. After 60 minutes of exposure, ioxaglate at the higher concentration (150 mg iodine per milliliter) significantly increased the LDH signal (ie, the percentage of LDH released), to 20{\%}. Conclusion. The present findings demonstrate that ioxaglate but not iohexol causes morphologic degeneration of the microvascular endothelial cells. This direct cytotoxic action of ioxaglate probably causes endothelial cell dysfunction, closely associated with the occurrence of phlebitis, pain, and thrombosis.",
keywords = "Contrast media, Effects, Experimental studies, Iohexol, Ioxaglate",
author = "W. Furuta and Toshiaki Sendo and Y. Kataoka and R. Oishi",
year = "2001",
language = "English",
volume = "8",
pages = "158--161",
journal = "Academic Radiology",
issn = "1076-6332",
publisher = "Elsevier USA",
number = "2",

}

TY - JOUR

T1 - Morphologic degeneration of human microvascular endothelial cells induced by iodinated contrast media

AU - Furuta, W.

AU - Sendo, Toshiaki

AU - Kataoka, Y.

AU - Oishi, R.

PY - 2001

Y1 - 2001

N2 - Rationale and Objectives. The purpose of this study was to characterize the adverse effects of iohexol and ioxaglate on human microvascular endothelial cells, which may result in phlebitis, pain, and thrombosis. Materials and Methods. The degree of morphologic degeneration and of lactate dehydrogenase (LDH) efflux into the extracellular medium (as an index of cell viability) were determined in endothelial cell culture exposed for 10, 30, or 60 minutes to ioxaglate or iohexol (ionic and nonionic contrast media, respectively) at iodine concentrations of 100 or 150 mg/mL. Results. Ioxaglate induced concentration- and time-dependent morphologic degeneration, including shrinkage and loss of the cell tip in 20%-80% of endothelial cells; iohexol did not. After 60 minutes of exposure, ioxaglate at the higher concentration (150 mg iodine per milliliter) significantly increased the LDH signal (ie, the percentage of LDH released), to 20%. Conclusion. The present findings demonstrate that ioxaglate but not iohexol causes morphologic degeneration of the microvascular endothelial cells. This direct cytotoxic action of ioxaglate probably causes endothelial cell dysfunction, closely associated with the occurrence of phlebitis, pain, and thrombosis.

AB - Rationale and Objectives. The purpose of this study was to characterize the adverse effects of iohexol and ioxaglate on human microvascular endothelial cells, which may result in phlebitis, pain, and thrombosis. Materials and Methods. The degree of morphologic degeneration and of lactate dehydrogenase (LDH) efflux into the extracellular medium (as an index of cell viability) were determined in endothelial cell culture exposed for 10, 30, or 60 minutes to ioxaglate or iohexol (ionic and nonionic contrast media, respectively) at iodine concentrations of 100 or 150 mg/mL. Results. Ioxaglate induced concentration- and time-dependent morphologic degeneration, including shrinkage and loss of the cell tip in 20%-80% of endothelial cells; iohexol did not. After 60 minutes of exposure, ioxaglate at the higher concentration (150 mg iodine per milliliter) significantly increased the LDH signal (ie, the percentage of LDH released), to 20%. Conclusion. The present findings demonstrate that ioxaglate but not iohexol causes morphologic degeneration of the microvascular endothelial cells. This direct cytotoxic action of ioxaglate probably causes endothelial cell dysfunction, closely associated with the occurrence of phlebitis, pain, and thrombosis.

KW - Contrast media

KW - Effects

KW - Experimental studies

KW - Iohexol

KW - Ioxaglate

UR - http://www.scopus.com/inward/record.url?scp=0034911698&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0034911698&partnerID=8YFLogxK

M3 - Article

C2 - 11227644

AN - SCOPUS:0034911698

VL - 8

SP - 158

EP - 161

JO - Academic Radiology

JF - Academic Radiology

SN - 1076-6332

IS - 2

ER -