TY - JOUR
T1 - Morpho-molecular characterization of causative agents of wilting, leaf spot, fruit blight and stem canker of okra (Abelmoschus esculentus L.)
AU - Hossain, Md Anowar
AU - Munshi, Arifur Rahman
AU - Hossen, Md Sajjad
AU - Rahman, Kazi Zahidur
AU - Karim, Md Rezaul
AU - Kimura, Yoshinobu
N1 - Funding Information:
This research work was funded by the Faculty of Science, University of Rajshahi (2018?2019) (Project no-1228/5/52RU/Scienc-19/2019-2020).
Publisher Copyright:
© 2021 Informa UK Limited, trading as Taylor & Francis Group.
PY - 2021
Y1 - 2021
N2 - This study aimed at identification and characterisation of the wilting, leaf spot, stem canker and fruit-blight diseases of okra. Pathogen infected plant samples were sterilised with 2% sodium hychlorite solution for 20 mins and then cultured at 28 °C for 15 days followed by microscopic examination. Combined microscopic, morphological and cultural characteristics analyses identified the four okra fungal isolates (OFI) such as OFI-1, OFI-2, OFI-3 and OFI-4 as Fusarium oxysporum, Curvularia lunata, Fusarium chlamydosporum and Choanephora cucurbitarum, respectively. The isolates OFI-1, OFI-2, OFI-4 showed highest radial growth rates at 25 °C, pH 5 and 20% lactose containing media while it was sucrose for OFI-3. BLASTN search using the sequence of ITS regions revealed that OFI-1, OFI-2, OFI-3 and OFI-4 isolates had 98, 100, 97 and 100% homology with F. oxysporum, C. lunata, F. chlamydosporum and C. cucurbitarum, respectively. Pathogenicity tests were also confirmed by reproducing the disease symptoms in okra plants.
AB - This study aimed at identification and characterisation of the wilting, leaf spot, stem canker and fruit-blight diseases of okra. Pathogen infected plant samples were sterilised with 2% sodium hychlorite solution for 20 mins and then cultured at 28 °C for 15 days followed by microscopic examination. Combined microscopic, morphological and cultural characteristics analyses identified the four okra fungal isolates (OFI) such as OFI-1, OFI-2, OFI-3 and OFI-4 as Fusarium oxysporum, Curvularia lunata, Fusarium chlamydosporum and Choanephora cucurbitarum, respectively. The isolates OFI-1, OFI-2, OFI-4 showed highest radial growth rates at 25 °C, pH 5 and 20% lactose containing media while it was sucrose for OFI-3. BLASTN search using the sequence of ITS regions revealed that OFI-1, OFI-2, OFI-3 and OFI-4 isolates had 98, 100, 97 and 100% homology with F. oxysporum, C. lunata, F. chlamydosporum and C. cucurbitarum, respectively. Pathogenicity tests were also confirmed by reproducing the disease symptoms in okra plants.
KW - Choanephora cucurbitarum
KW - Curvularia lunata
KW - Fusarium sp
KW - morphology
KW - pathogenicity
KW - phylogenetics
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U2 - 10.1080/03235408.2021.1917283
DO - 10.1080/03235408.2021.1917283
M3 - Article
AN - SCOPUS:85105445747
VL - 54
SP - 1501
EP - 1518
JO - Archives of Phytopathology and Plant Protection
JF - Archives of Phytopathology and Plant Protection
SN - 0323-5408
IS - 17-18
ER -