Monocyte chemoattractant protein-1 in idiopathic pulmonary fibrosis and other interstitial lung diseases

Kazuhiro Iyonaga, Motohiro Takeya, Naoki Saita, Osamu Sakamoto, Teizo Yoshimura, Masayuki Ando, Kiyoshi Takahashi

Research output: Contribution to journalArticle

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Abstract

Macrophages play a crucial role in the pathogenesis of idiopathic pulmonary fibrosis (IPF). To examine the mechanisms for increased monocyte/macrophage recruitment in IPF and nonIPF interstitial lung diseases (nonIPF) the localization of monocyte chemoattractant protein-1 (MCP-1) was investigated in 14 cases of IPF, seven cases of nonIPF, and seven normal control lungs (CTRL) by immunohistochemistry using a specific anti-MCP-1 monoclonal antibody, F9. By double immunohistochemical staining using F9 and one of the cell type specific antibodies significant differences in the staining pattern of MCP-1 were observed between IPF and nonIPF. In IPF MCP-1 was observed in cuboidal and flattened metaplastic epithelial cells, alveolar macrophages, and vascular endothelial cells. In contrast, no epithelial cells were stained for MCP-1 in nonIPF cases, although alveolar macrophages and vascular endothelial cells were labeled. Northern hybridization analysis of selected cases showed marked expression of MCP-1 messenger RNA (mRNA) in IPF and nonIPF compared with CTRL. These findings suggest that the MCP-1 production in IPF and nonIPF plays an important role in the recruitment of monocyte/macrophages. Monocyte chemoattractant protein-1 production by epithelial cells in IPF may be caused by the metaplastic nature of the epithelial cells and may be one of the key factors inducing the irreversible progression of IPF.

Original languageEnglish
Pages (from-to)455-463
Number of pages9
JournalHuman Pathology
Volume25
Issue number5
DOIs
Publication statusPublished - 1994
Externally publishedYes

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Idiopathic Pulmonary Fibrosis
Chemokine CCL2
Interstitial Lung Diseases
Epithelial Cells
Macrophages
Alveolar Macrophages
Monocytes
Endothelial Cells
Staining and Labeling
Lung
Immunohistochemistry
Monoclonal Antibodies
Messenger RNA
Antibodies

Keywords

  • immunohistochemistry
  • MCP-1
  • metaplastic epithelial cells
  • monoclonal antibody
  • Northern blot analysis

ASJC Scopus subject areas

  • Pathology and Forensic Medicine

Cite this

Monocyte chemoattractant protein-1 in idiopathic pulmonary fibrosis and other interstitial lung diseases. / Iyonaga, Kazuhiro; Takeya, Motohiro; Saita, Naoki; Sakamoto, Osamu; Yoshimura, Teizo; Ando, Masayuki; Takahashi, Kiyoshi.

In: Human Pathology, Vol. 25, No. 5, 1994, p. 455-463.

Research output: Contribution to journalArticle

Iyonaga, Kazuhiro ; Takeya, Motohiro ; Saita, Naoki ; Sakamoto, Osamu ; Yoshimura, Teizo ; Ando, Masayuki ; Takahashi, Kiyoshi. / Monocyte chemoattractant protein-1 in idiopathic pulmonary fibrosis and other interstitial lung diseases. In: Human Pathology. 1994 ; Vol. 25, No. 5. pp. 455-463.
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