Monoclonal suncus antibodies: Generation of fusion partners to produce suncus-suncus hybridomas

Yoshikazu Sado, Satoko Inoue, Yasuko Tomono, Makoto Matsuyama, Masaki Fukushima, Toshitaka Oohashi, Takamichi Jogahara, Sen Ichi Oda

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

We used suncus (Suncus murinus; house musk shrew) to generate partner cells for cell fusion to produce suncus monoclonal antibodies. Suncus are insectivores that are genetically distant to rodents, and recognize antigens and epitopes that are not immunogenic in mice and rats, which are the animals most commonly used in basic life science research and from which monoclonal antibodies are usually produced. To date, monoclonal antibodies from suncus have not been generated due to the lack of a plasmacytoma fusion partner. To obtain suncus plasmacytoma cell lines suitable as a cell fusion partner, we injected suncus at both sides of the tail base with antigen emulsion, collected the lymph nodes and spleens, and cultured the cells to obtain immortalized lymphoid cell lines visually resembling mouse SP2/0-Ag14 myeloma cells. Three suncus immunized with the antigen provided 4 cell lines of suncus plasmacytoma, but they did not secrete immunoglobulins. Antibody-producing hybrid cells were generated from these cell lines using a cell fusion technique. Using one of the cell lines as a fusion partner, we obtained six lines of immunoglobulin-producing hybrid cells which secreted an unidentified monoclonal IgG. When these 6 lines were used as new fusion partners, we obtained several hybrid cell lines which secreted immunogen-specific monoclonal antibodies. These hybrid cells can be cloned and cryopreserved. We also obtained another good fusion partner which initially secreted antibody but later stopped doing so. These suncus-suncus hybrid cell lines will be useful for the production of suncus monoclonal antibodies.

Original languageEnglish
Pages (from-to)71-84
Number of pages14
JournalACTA HISTOCHEMICA ET CYTOCHEMICA
Volume50
Issue number2
DOIs
Publication statusPublished - 2017

Keywords

  • Cell fusion
  • Fusion partner cell
  • Lymph node
  • Suncus monoclonal antibody

ASJC Scopus subject areas

  • Pathology and Forensic Medicine
  • Biochemistry
  • Physiology
  • Histology
  • Cell Biology

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  • Cite this

    Sado, Y., Inoue, S., Tomono, Y., Matsuyama, M., Fukushima, M., Oohashi, T., Jogahara, T., & Oda, S. I. (2017). Monoclonal suncus antibodies: Generation of fusion partners to produce suncus-suncus hybridomas. ACTA HISTOCHEMICA ET CYTOCHEMICA, 50(2), 71-84. https://doi.org/10.1267/ahc.17007