Mono‐ADP‐Ribosylation of Arginine Residue of Euglena gracilis Z in Synchronous Culture

SHIGEO TAKENAK; JUNKO INAGAKI; SHINGO TSUYAMA; KAZUTAKA MIYATAKE; YOSHIHISA NAKANO

doi:10.1111/j.1550-7408.1995.tb01596.x

Mono‐ADP‐Ribosylation of Arginine Residue of Euglena gracilis Z in Synchronous Culture

SHIGEO TAKENAK, JUNKO INAGAKI, SHINGO TSUYAMA, KAZUTAKA MIYATAKE, YOSHIHISA NAKANO

Research output: Contribution to journal › Article › peer-review

2 Citations (Scopus)

Abstract

ABSTRACT. In Euglena gracilis Z, a considerably high activity of mono‐ADP‐ribosyltransferase occurred and change of it was accompanied by a cell cycle induced by a light‐dark cycle. The enzyme activity was strongly inhibited by L‐arginine and supported in the presence of poly‐L‐arginine as a substrate, indicating that ADP‐ribosylated amino acid is an arginine residue. Arginine: mono‐ADP‐ribosyltransferase activity was found in the chloroplasts, mitochondria, microsomes and cytosol as judged from marker enzyme activities and the activity in each organelle fluctuated with the cell cycle.

Original language	English
Pages (from-to)	373-376
Number of pages	4
Journal	Journal of Eukaryotic Microbiology
Volume	42
Issue number	4
DOIs	https://doi.org/10.1111/j.1550-7408.1995.tb01596.x
Publication status	Published - Jul 1995

Keywords

Cell cycle
Euglena gracilis Z
NAD
cytokinesis
organelles organellogenesis

ASJC Scopus subject areas

Microbiology

Access to Document

10.1111/j.1550-7408.1995.tb01596.x

Cite this

@article{b156d0a26687409bbb607bf3490d6487,

title = "Mono‐ADP‐Ribosylation of Arginine Residue of Euglena gracilis Z in Synchronous Culture",

abstract = "ABSTRACT. In Euglena gracilis Z, a considerably high activity of mono‐ADP‐ribosyltransferase occurred and change of it was accompanied by a cell cycle induced by a light‐dark cycle. The enzyme activity was strongly inhibited by L‐arginine and supported in the presence of poly‐L‐arginine as a substrate, indicating that ADP‐ribosylated amino acid is an arginine residue. Arginine: mono‐ADP‐ribosyltransferase activity was found in the chloroplasts, mitochondria, microsomes and cytosol as judged from marker enzyme activities and the activity in each organelle fluctuated with the cell cycle.",

keywords = "Cell cycle, Euglena gracilis Z, NAD, cytokinesis, organelles organellogenesis",

author = "SHIGEO TAKENAK and JUNKO INAGAKI and SHINGO TSUYAMA and KAZUTAKA MIYATAKE and YOSHIHISA NAKANO",

year = "1995",

month = jul,

doi = "10.1111/j.1550-7408.1995.tb01596.x",

language = "English",

volume = "42",

pages = "373--376",

journal = "Journal of Protozoology",

issn = "1066-5234",

publisher = "Wiley-Blackwell",

number = "4",

}

TY - JOUR

T1 - Mono‐ADP‐Ribosylation of Arginine Residue of Euglena gracilis Z in Synchronous Culture

AU - TAKENAK, SHIGEO

AU - INAGAKI, JUNKO

AU - TSUYAMA, SHINGO

AU - MIYATAKE, KAZUTAKA

AU - NAKANO, YOSHIHISA

PY - 1995/7

Y1 - 1995/7

N2 - ABSTRACT. In Euglena gracilis Z, a considerably high activity of mono‐ADP‐ribosyltransferase occurred and change of it was accompanied by a cell cycle induced by a light‐dark cycle. The enzyme activity was strongly inhibited by L‐arginine and supported in the presence of poly‐L‐arginine as a substrate, indicating that ADP‐ribosylated amino acid is an arginine residue. Arginine: mono‐ADP‐ribosyltransferase activity was found in the chloroplasts, mitochondria, microsomes and cytosol as judged from marker enzyme activities and the activity in each organelle fluctuated with the cell cycle.

AB - ABSTRACT. In Euglena gracilis Z, a considerably high activity of mono‐ADP‐ribosyltransferase occurred and change of it was accompanied by a cell cycle induced by a light‐dark cycle. The enzyme activity was strongly inhibited by L‐arginine and supported in the presence of poly‐L‐arginine as a substrate, indicating that ADP‐ribosylated amino acid is an arginine residue. Arginine: mono‐ADP‐ribosyltransferase activity was found in the chloroplasts, mitochondria, microsomes and cytosol as judged from marker enzyme activities and the activity in each organelle fluctuated with the cell cycle.

KW - Cell cycle

KW - Euglena gracilis Z

KW - NAD

KW - cytokinesis

KW - organelles organellogenesis

UR - http://www.scopus.com/inward/record.url?scp=0028869972&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0028869972&partnerID=8YFLogxK

U2 - 10.1111/j.1550-7408.1995.tb01596.x

DO - 10.1111/j.1550-7408.1995.tb01596.x

M3 - Article

AN - SCOPUS:0028869972

SN - 1066-5234

VL - 42

SP - 373

EP - 376

JO - Journal of Protozoology

JF - Journal of Protozoology

IS - 4

ER -

Mono‐ADP‐Ribosylation of Arginine Residue of Euglena gracilis Z in Synchronous Culture

Abstract

Keywords

ASJC Scopus subject areas

Access to Document

Other files and links

Fingerprint

Cite this