Monoacylglycerols: Glycolipid biosurfactants produced by a thermotolerant yeast, Candida ishiwadae

B. Thanomsub, T. Watcharachaipong, K. Chotelersak, P. Arunrattiyakorn, T. Nitoda, H. Kanzaki

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38 Citations (Scopus)

Abstract

Aims: To isolate and characterize biosurfactants produced by a thermotolerant yeast isolated in Thailand. Materials and Results: Yeast strains isolated from plant material in Thailand were first screened for the ability to produce lipase and biosurfactant. A strain Y12, identified as Candida ishiwadae by physiological tests, survived at 45°C and produced relatively large amounts of biosurfactants. From the culture filtrate of this strain, two glycolipid biosurfactants, a and b, were purified by solvent fractionation, silica gel and ODS column chromatographies. Compounds a and b were determined to be monoacylglycerols; 1-linoleylglycerol and 1-oleylglycerol, respectively. Both compounds exhibited higher surfactant activities tested by the drop collapse test than several artificial surfactants such as sodium dodecyl sulphate. Conclusions: Glycolipid biosurfactants produced by a thermotolerant yeast, C. ishiwadae were characterized to be monoacylglycerols which exhibited high surfactant activities. Significance and Impact of the Study: A thermotolerant yeast strain, C. ishiwadae, could be a potential candidate for producing monoacylglycerols which are useful in industrial applications.

Original languageEnglish
Pages (from-to)588-592
Number of pages5
JournalJournal of Applied Microbiology
Volume96
Issue number3
DOIs
Publication statusPublished - Mar 16 2004

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Keywords

  • 1-acylglycerol
  • Biosurfactant
  • Glycolipid
  • Lipase
  • Thermotolerant
  • Yeast

ASJC Scopus subject areas

  • Biotechnology
  • Applied Microbiology and Biotechnology

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