TY - JOUR
T1 - Monitoring remineralization of enamel subsurface lesions by optical coherence tomography
AU - Mandurah, Mona M.
AU - Sadr, Alireza
AU - Shimada, Yasushi
AU - Kitasako, Yuichi
AU - Nakashima, Syozi
AU - Bakhsh, Turki A.
AU - Tagami, Junji
AU - Sumi, Yasunori
N1 - Funding Information:
This work was supported by a grant from Japanese Ministry of education, Global Center of Excellence Program (GCOE), International Research Center for Molecular Science in Tooth and Bone Diseases; the Research Grant for Longevity Sciences (21A-8) from Ministry of Health, Labor, and Welfare; and Grant-in-Aid for Scientific Research (Nos. 23659886 and 24792019) from the Japan Society for the Promotion of Science (JSPS). Authors are also grateful to Drs. Ilnaz Hariri and Sahar Khunkar for their assistance. POs-Ca was donated by Ezaki Glico company for this research.
PY - 2013/4
Y1 - 2013/4
N2 - Optical coherence tomography (OCT) is a potential clinical tool for enamel lesion monitoring. Sweptsource OCT findings were compared with cross-sectional nanohardness findings of enamel. Subsurface bovine enamel lesions in three groups were subjected to (1) deionized water (control), (2) phosphoryl oligosaccharide of calcium (POs-Ca) or (3) POs-Ca with 1 ppm fluoride for 14 days. B-scans images were obtained at 1310-nm center wavelength on sound, demineralized and remineralized areas after 4, 7, and 14 days. The specimens were processed for cross-sectional nanoindentation. Reflectivity from enamel that had increased with demineralization decreased with remineralization. An OCT attenuation coefficient parameter (μt), derived based on the Beer-Lambert law as a function of backscatter signal slope, showed a strong linear regression with integrated nanohardness of all regions (p < 0.001, r = ?0.97). Sound enamel showed the smallest, while demineralized enamel showed the highest μt. In group three, μt was significantly lower at four days than baseline, but remained constant afterwards. In group two, the changes were rather gradual. There was no significant difference between groups two and three at 14 days in nanohardness or μt POs-Ca with fluoride-enhanced nanohardness of the superficial zone. OCT signal attenuation demonstrated a capability for monitoring changes of enamel lesions during remineralization.
AB - Optical coherence tomography (OCT) is a potential clinical tool for enamel lesion monitoring. Sweptsource OCT findings were compared with cross-sectional nanohardness findings of enamel. Subsurface bovine enamel lesions in three groups were subjected to (1) deionized water (control), (2) phosphoryl oligosaccharide of calcium (POs-Ca) or (3) POs-Ca with 1 ppm fluoride for 14 days. B-scans images were obtained at 1310-nm center wavelength on sound, demineralized and remineralized areas after 4, 7, and 14 days. The specimens were processed for cross-sectional nanoindentation. Reflectivity from enamel that had increased with demineralization decreased with remineralization. An OCT attenuation coefficient parameter (μt), derived based on the Beer-Lambert law as a function of backscatter signal slope, showed a strong linear regression with integrated nanohardness of all regions (p < 0.001, r = ?0.97). Sound enamel showed the smallest, while demineralized enamel showed the highest μt. In group three, μt was significantly lower at four days than baseline, but remained constant afterwards. In group two, the changes were rather gradual. There was no significant difference between groups two and three at 14 days in nanohardness or μt POs-Ca with fluoride-enhanced nanohardness of the superficial zone. OCT signal attenuation demonstrated a capability for monitoring changes of enamel lesions during remineralization.
KW - Beer-Lambert law
KW - attenuation coefficient
KW - enamel subsurface lesion
KW - integrated nanohardness
KW - nanoindentation
KW - optical coherence tomography
KW - phosphoryl oligosaccharide of calcium
KW - remineralization
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U2 - 10.1117/1.JBO.18.4.046006
DO - 10.1117/1.JBO.18.4.046006
M3 - Article
C2 - 23563920
AN - SCOPUS:84884193838
VL - 18
JO - Journal of Biomedical Optics
JF - Journal of Biomedical Optics
SN - 1083-3668
IS - 4
M1 - 046006
ER -