Abstract
Herpesviruses frequently cause serious complications after allogeneic bone marrow transplantation (allo-BMT). Recent studies have shown more rapid immune reconstitution after allogeneic peripheral blood stem cell transplantation (allo-PBSCT) compared with allo-BMT. However, it has not been clarified whether the improved immune reconstitution after allo-PBSCT is associated with a lower incidence of herpesvirus infections. We monitored the emergence of Epstein-Barr virus (EBV), cytomegalovirus (CMV), human herpesvirus 6 (HHV-6) and HHV-7 DNA by a nested-double polymerase chain reaction in peripheral blood leucocytes from 22 allo-BMT and 16 allo-PBSCT patients. Each virus had an unique temporal profile of detection. HHV-6 DNA was detected most frequently at 3 weeks after transplantation, whereas CMV and EBV DNA were detected later (2-3 months). Detection rates of HHV-6 DNA at 3 and 4 weeks after allo-BMT were significantly higher than those after allo- PBSCT (9/16 v 2/13 at 3 weeks. P <0.01; 10/21 v 1/15 at 4 weeks, P <0.01). Detection rates of the other three herpesviruses after the two types of allogeneic transplantation were not significantly different throughout observation period. Furthermore, detection of HHV-6 DNA within the first 4 weeks was associated with delayed platelet engraftment after both allo-BMT and allo-PBSCT (P
| Original language | English |
|---|---|
| Pages (from-to) | 295-302 |
| Number of pages | 8 |
| Journal | British Journal of Haematology |
| Volume | 105 |
| Issue number | 1 |
| DOIs | |
| Publication status | Published - 1999 |
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Keywords
- Allogeneic PBSCT
- CMV
- Engraftment
- Herpesvirus
- HHV-6
ASJC Scopus subject areas
- Hematology
Cite this
Monitoring of human herpesviruses after allogeneic peripheral blood stem cell transplantation and bone marrow transplantation. / Maeda, Yoshinobu; Teshima, Takanori; Yamada, Masao; Shinagawa, Katsuji; Nakao, Shinji; Ohno, Yuju; Kojima, Kensuke; Hara, Masamichi; Nagafuji, Koji; Hayashi, Shin; Fukuda, Shunnichi; Sawada, Hitoshi; Matsue, Kosei; Takenaka, Katsuto; Ishimaru, Fumihiko; Ikeda, Kazuma; Niiya, Kenji; Harada, Mine.
In: British Journal of Haematology, Vol. 105, No. 1, 1999, p. 295-302.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Monitoring of human herpesviruses after allogeneic peripheral blood stem cell transplantation and bone marrow transplantation
AU - Maeda, Yoshinobu
AU - Teshima, Takanori
AU - Yamada, Masao
AU - Shinagawa, Katsuji
AU - Nakao, Shinji
AU - Ohno, Yuju
AU - Kojima, Kensuke
AU - Hara, Masamichi
AU - Nagafuji, Koji
AU - Hayashi, Shin
AU - Fukuda, Shunnichi
AU - Sawada, Hitoshi
AU - Matsue, Kosei
AU - Takenaka, Katsuto
AU - Ishimaru, Fumihiko
AU - Ikeda, Kazuma
AU - Niiya, Kenji
AU - Harada, Mine
PY - 1999
Y1 - 1999
N2 - Herpesviruses frequently cause serious complications after allogeneic bone marrow transplantation (allo-BMT). Recent studies have shown more rapid immune reconstitution after allogeneic peripheral blood stem cell transplantation (allo-PBSCT) compared with allo-BMT. However, it has not been clarified whether the improved immune reconstitution after allo-PBSCT is associated with a lower incidence of herpesvirus infections. We monitored the emergence of Epstein-Barr virus (EBV), cytomegalovirus (CMV), human herpesvirus 6 (HHV-6) and HHV-7 DNA by a nested-double polymerase chain reaction in peripheral blood leucocytes from 22 allo-BMT and 16 allo-PBSCT patients. Each virus had an unique temporal profile of detection. HHV-6 DNA was detected most frequently at 3 weeks after transplantation, whereas CMV and EBV DNA were detected later (2-3 months). Detection rates of HHV-6 DNA at 3 and 4 weeks after allo-BMT were significantly higher than those after allo- PBSCT (9/16 v 2/13 at 3 weeks. P <0.01; 10/21 v 1/15 at 4 weeks, P <0.01). Detection rates of the other three herpesviruses after the two types of allogeneic transplantation were not significantly different throughout observation period. Furthermore, detection of HHV-6 DNA within the first 4 weeks was associated with delayed platelet engraftment after both allo-BMT and allo-PBSCT (P
AB - Herpesviruses frequently cause serious complications after allogeneic bone marrow transplantation (allo-BMT). Recent studies have shown more rapid immune reconstitution after allogeneic peripheral blood stem cell transplantation (allo-PBSCT) compared with allo-BMT. However, it has not been clarified whether the improved immune reconstitution after allo-PBSCT is associated with a lower incidence of herpesvirus infections. We monitored the emergence of Epstein-Barr virus (EBV), cytomegalovirus (CMV), human herpesvirus 6 (HHV-6) and HHV-7 DNA by a nested-double polymerase chain reaction in peripheral blood leucocytes from 22 allo-BMT and 16 allo-PBSCT patients. Each virus had an unique temporal profile of detection. HHV-6 DNA was detected most frequently at 3 weeks after transplantation, whereas CMV and EBV DNA were detected later (2-3 months). Detection rates of HHV-6 DNA at 3 and 4 weeks after allo-BMT were significantly higher than those after allo- PBSCT (9/16 v 2/13 at 3 weeks. P <0.01; 10/21 v 1/15 at 4 weeks, P <0.01). Detection rates of the other three herpesviruses after the two types of allogeneic transplantation were not significantly different throughout observation period. Furthermore, detection of HHV-6 DNA within the first 4 weeks was associated with delayed platelet engraftment after both allo-BMT and allo-PBSCT (P
KW - Allogeneic PBSCT
KW - CMV
KW - Engraftment
KW - Herpesvirus
KW - HHV-6
UR - http://www.scopus.com/inward/record.url?scp=0032917334&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0032917334&partnerID=8YFLogxK
U2 - 10.1111/j.1365-2141.1999.01290.x
DO - 10.1111/j.1365-2141.1999.01290.x
M3 - Article
VL - 105
SP - 295
EP - 302
JO - British Journal of Haematology
JF - British Journal of Haematology
SN - 0007-1048
IS - 1
ER -