Monitoring of human herpesviruses-6 and -7 DNA in saliva samples during the acute and convalescent phases of exanthem subitum

Yuki Miyazaki, Hikaru Namba, Sadayoshi Torigoe, Masahiro Watanabe, Nobuko Yamashita, Hirohito Ogawa, Tsuneo Morishima, Masao Yamada

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

The amounts of the DNAs of human herpesviruses-6 (HHV-6) and -7 (HHV-7) in saliva samples were monitored during the acute and convalescent phases of exanthem subitum (ES) to elucidate the kinetics of virus shedding after ES. A total of 247 saliva samples were collected from 17 children (5 males and 12 females: 8-31 months old at onset). The monitoring period ranged from 152 to 721 days after onset, and in 15 children it was longer than 1 year. Among the 17 cases, 16 were attributed to HHV-6B, while a single case was attributed to HHV-7. Detection rates and average amounts of HHV-6 DNA in saliva samples after ES attributed to HHV-6B were low in the acute phase, increased to the maximum in the convalescent phase at 3-7 months, and then decreased. In addition, to investigate the source of infection, saliva samples from the older siblings (age 3-9 years) and parents of ES patients and children with a history of ES were also examined. The detection rate of HHV-6 DNA in saliva samples from 3- to 9-year-old children was significantly higher than the rate in adult saliva samples. Taken together, these findings suggest that the saliva of children in the convalescent phase of ES might be a more likely source of HHV-6 infection than that of adults. J. Med. Virol. © 2016 Wiley Periodicals, Inc.

Original languageEnglish
JournalJournal of Medical Virology
DOIs
Publication statusPublished - Sep 20 2016
Externally publishedYes

Fingerprint

Human Herpesvirus 7
Human Herpesvirus 6
Exanthema
Saliva
DNA
Virus Shedding
Herpesviridae Infections
Siblings
Parents

Cite this

Monitoring of human herpesviruses-6 and -7 DNA in saliva samples during the acute and convalescent phases of exanthem subitum. / Miyazaki, Yuki; Namba, Hikaru; Torigoe, Sadayoshi; Watanabe, Masahiro; Yamashita, Nobuko; Ogawa, Hirohito; Morishima, Tsuneo; Yamada, Masao.

In: Journal of Medical Virology, 20.09.2016.

Research output: Contribution to journalArticle

@article{835f015b18ec4466bf1697ffccddb0f5,
title = "Monitoring of human herpesviruses-6 and -7 DNA in saliva samples during the acute and convalescent phases of exanthem subitum",
abstract = "The amounts of the DNAs of human herpesviruses-6 (HHV-6) and -7 (HHV-7) in saliva samples were monitored during the acute and convalescent phases of exanthem subitum (ES) to elucidate the kinetics of virus shedding after ES. A total of 247 saliva samples were collected from 17 children (5 males and 12 females: 8-31 months old at onset). The monitoring period ranged from 152 to 721 days after onset, and in 15 children it was longer than 1 year. Among the 17 cases, 16 were attributed to HHV-6B, while a single case was attributed to HHV-7. Detection rates and average amounts of HHV-6 DNA in saliva samples after ES attributed to HHV-6B were low in the acute phase, increased to the maximum in the convalescent phase at 3-7 months, and then decreased. In addition, to investigate the source of infection, saliva samples from the older siblings (age 3-9 years) and parents of ES patients and children with a history of ES were also examined. The detection rate of HHV-6 DNA in saliva samples from 3- to 9-year-old children was significantly higher than the rate in adult saliva samples. Taken together, these findings suggest that the saliva of children in the convalescent phase of ES might be a more likely source of HHV-6 infection than that of adults. J. Med. Virol. {\circledC} 2016 Wiley Periodicals, Inc.",
author = "Yuki Miyazaki and Hikaru Namba and Sadayoshi Torigoe and Masahiro Watanabe and Nobuko Yamashita and Hirohito Ogawa and Tsuneo Morishima and Masao Yamada",
note = "{\circledC} 2016 Wiley Periodicals, Inc.",
year = "2016",
month = "9",
day = "20",
doi = "10.1002/jmv.24690",
language = "English",
journal = "Journal of Medical Virology",
issn = "0146-6615",
publisher = "Wiley-Liss Inc.",

}

TY - JOUR

T1 - Monitoring of human herpesviruses-6 and -7 DNA in saliva samples during the acute and convalescent phases of exanthem subitum

AU - Miyazaki, Yuki

AU - Namba, Hikaru

AU - Torigoe, Sadayoshi

AU - Watanabe, Masahiro

AU - Yamashita, Nobuko

AU - Ogawa, Hirohito

AU - Morishima, Tsuneo

AU - Yamada, Masao

N1 - © 2016 Wiley Periodicals, Inc.

PY - 2016/9/20

Y1 - 2016/9/20

N2 - The amounts of the DNAs of human herpesviruses-6 (HHV-6) and -7 (HHV-7) in saliva samples were monitored during the acute and convalescent phases of exanthem subitum (ES) to elucidate the kinetics of virus shedding after ES. A total of 247 saliva samples were collected from 17 children (5 males and 12 females: 8-31 months old at onset). The monitoring period ranged from 152 to 721 days after onset, and in 15 children it was longer than 1 year. Among the 17 cases, 16 were attributed to HHV-6B, while a single case was attributed to HHV-7. Detection rates and average amounts of HHV-6 DNA in saliva samples after ES attributed to HHV-6B were low in the acute phase, increased to the maximum in the convalescent phase at 3-7 months, and then decreased. In addition, to investigate the source of infection, saliva samples from the older siblings (age 3-9 years) and parents of ES patients and children with a history of ES were also examined. The detection rate of HHV-6 DNA in saliva samples from 3- to 9-year-old children was significantly higher than the rate in adult saliva samples. Taken together, these findings suggest that the saliva of children in the convalescent phase of ES might be a more likely source of HHV-6 infection than that of adults. J. Med. Virol. © 2016 Wiley Periodicals, Inc.

AB - The amounts of the DNAs of human herpesviruses-6 (HHV-6) and -7 (HHV-7) in saliva samples were monitored during the acute and convalescent phases of exanthem subitum (ES) to elucidate the kinetics of virus shedding after ES. A total of 247 saliva samples were collected from 17 children (5 males and 12 females: 8-31 months old at onset). The monitoring period ranged from 152 to 721 days after onset, and in 15 children it was longer than 1 year. Among the 17 cases, 16 were attributed to HHV-6B, while a single case was attributed to HHV-7. Detection rates and average amounts of HHV-6 DNA in saliva samples after ES attributed to HHV-6B were low in the acute phase, increased to the maximum in the convalescent phase at 3-7 months, and then decreased. In addition, to investigate the source of infection, saliva samples from the older siblings (age 3-9 years) and parents of ES patients and children with a history of ES were also examined. The detection rate of HHV-6 DNA in saliva samples from 3- to 9-year-old children was significantly higher than the rate in adult saliva samples. Taken together, these findings suggest that the saliva of children in the convalescent phase of ES might be a more likely source of HHV-6 infection than that of adults. J. Med. Virol. © 2016 Wiley Periodicals, Inc.

U2 - 10.1002/jmv.24690

DO - 10.1002/jmv.24690

M3 - Article

C2 - 27648817

JO - Journal of Medical Virology

JF - Journal of Medical Virology

SN - 0146-6615

ER -