Molecular screening of anti-quorum sensing capability of Salvadora persica on Enterococcus faecalis

Afsaneh Rezaei, Glenn G. Oyong, Virgilio B. Borja, Masahisa Inoue, Tomomi Abe, Ryo Tamamura, Hitoshi Nagatsuka, Kojun Setsu, Rosario R. Buery

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

Quorum sensing (QS) refers to the phenomenon characterized by the accumulation of signaling molecules enabling a cell to sense a bacterial population triggering a coordinated response. Enterococcus faecalis is a resistant microorganism commonly found in oral infections. Salvadora persica has been traditionally used in Middle East countries to clean the teeth due to its antimicrobial activity. The study investigated the anti-quorum sensing (AQS) ability of S. persica extracts on E. faecalis. Extracts from the bark, leaves, root and shoots were tested against Chromobacterium violaceum. AQS ability was determined using differential scanning fluorimetry (DSF) and elastolytic assay to detect the level of protease and quantitative polymerase chain reaction (QPCR) to detect cytolysin (cylR1) and gelatinase (gelE) virulence factors. Preliminary disk diffusion assays revealed AQS activity by inhibiting violacein pigment production in C. violaceum without affecting its growth. Furthermore, only negligible amount of proteases was detected in DSF and elastolytic assays. CylR1 and gelE gene expression revealed no detectable signals even from the lowest possible threshold. The results indicate that S. persica has AQS ability against E. faecalis. S. persica extract can be used as alternative or in combination with other anti-microbial agents against infections caused by E. faecalis.

Original languageEnglish
Pages (from-to)115-124
Number of pages10
JournalJournal of Hard Tissue Biology
Volume20
Issue number2
DOIs
Publication statusPublished - Oct 20 2011

Keywords

  • Anti-quorum sensing
  • Enterococcus faecalis
  • Protease
  • Salvadora persica
  • Virulence factor

ASJC Scopus subject areas

  • Medicine (miscellaneous)
  • Biochemistry
  • Biomaterials
  • Orthopedics and Sports Medicine
  • Dentistry(all)
  • Cell Biology

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  • Cite this

    Rezaei, A., Oyong, G. G., Borja, V. B., Inoue, M., Abe, T., Tamamura, R., Nagatsuka, H., Setsu, K., & Buery, R. R. (2011). Molecular screening of anti-quorum sensing capability of Salvadora persica on Enterococcus faecalis. Journal of Hard Tissue Biology, 20(2), 115-124. https://doi.org/10.2485/jhtb.20.115