TY - JOUR
T1 - Molecular organizations and function of iron-stress-induced-A protein family in Anabaena sp. PCC 7120
AU - Nagao, Ryo
AU - Yokono, Makio
AU - Ueno, Yoshifumi
AU - Suzuki, Takehiro
AU - Kato, Koji
AU - Kato, Ka Ho
AU - Tsuboshita, Naoki
AU - Jiang, Tian Yi
AU - Dohmae, Naoshi
AU - Shen, Jian Ren
AU - Ehira, Shigeki
AU - Akimoto, Seiji
N1 - Funding Information:
This work was supported by JSPS KAKENHI No. JP19H04726 , JP20K06528 , JP20H02914 (to R. N.), JP20H02914 (K. K.), JP17H06433 (to J.-R. S.), JP19K22290 (to S. E.), and JP16H06553 (to S. A.).
PY - 2021/1/1
Y1 - 2021/1/1
N2 - Iron-stress-induced-A proteins (IsiAs) are expressed in cyanobacteria under iron-deficient conditions, and surround photosystem I (PSI) trimer with a ring formation. A cyanobacterium Anabaena sp. PCC 7120 has four isiA genes; however, it is unknown how the IsiAs are associated with PSI. Here we report on molecular organizations and function of the IsiAs in this cyanobacterium. A deletion mutant of the isiA1 gene was constructed, and the four types of thylakoids were prepared from the wild-type (WT) and ΔisiA1 cells under iron-replete (+Fe) and iron-deficient (−Fe) conditions. Immunoblotting analysis exhibits a clear expression of the IsiA1 in the WT−Fe. The PSI-IsiA1 supercomplex is found in the WT−Fe, and excitation-energy transfer from IsiA1 to PSI is verified by time-resolved fluorescence analyses. Instead of the IsiA1, both IsiA2 and IsiA3 are bound to PSI monomer in the ΔisiA1−Fe. These findings provide insights into multiple-expression system of the IsiA family in this cyanobacterium.
AB - Iron-stress-induced-A proteins (IsiAs) are expressed in cyanobacteria under iron-deficient conditions, and surround photosystem I (PSI) trimer with a ring formation. A cyanobacterium Anabaena sp. PCC 7120 has four isiA genes; however, it is unknown how the IsiAs are associated with PSI. Here we report on molecular organizations and function of the IsiAs in this cyanobacterium. A deletion mutant of the isiA1 gene was constructed, and the four types of thylakoids were prepared from the wild-type (WT) and ΔisiA1 cells under iron-replete (+Fe) and iron-deficient (−Fe) conditions. Immunoblotting analysis exhibits a clear expression of the IsiA1 in the WT−Fe. The PSI-IsiA1 supercomplex is found in the WT−Fe, and excitation-energy transfer from IsiA1 to PSI is verified by time-resolved fluorescence analyses. Instead of the IsiA1, both IsiA2 and IsiA3 are bound to PSI monomer in the ΔisiA1−Fe. These findings provide insights into multiple-expression system of the IsiA family in this cyanobacterium.
KW - Anabaena sp. PCC 7120
KW - Iron deficient
KW - IsiA
KW - Time-resolved fluorescence
UR - http://www.scopus.com/inward/record.url?scp=85094576815&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=85094576815&partnerID=8YFLogxK
U2 - 10.1016/j.bbabio.2020.148327
DO - 10.1016/j.bbabio.2020.148327
M3 - Article
C2 - 33069682
AN - SCOPUS:85094576815
VL - 1862
JO - Biochimica et Biophysica Acta - Bioenergetics
JF - Biochimica et Biophysica Acta - Bioenergetics
SN - 0005-2728
IS - 1
M1 - 148327
ER -