Molecular Monitoring of the Main Changes in Bacterial Floral Diversity in the Gastrointestinal Tract of a Thoroughbred Foal with Catarrhal Enteritis by Using PCR-DGGE

Hidetoshi Morita, Fumihiko Nakajima, Masaru Murakami, Akihito Endo, Takehito Suzuki, Chiharu Shiratori, Yukio Kato, Alexandre T. Okatani, Hiroaki Akita, Toshio Masaoka

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

In this study, the main changes in bacterial floral diversity in the gastrointestinal tract of a Thoroughbred foal were monitored by using polymerase chain reaction denaturing gradient gel electrophoresis (PCR-DGGE). The foal died of catarrhal enteritis of the cecum and large colon. Diarrheal feces and gastrointestinal contents were compared with normal feces. The closest relatives of the bacterium in the samples were Lactobacillus johnsonii (100% similarity), uncultured Bacteroides sp. (92.5% similarity), Bacteroides fragilis (96.3% similarity), and Enterococcus faecium/Enterococcus durans (100% similarity); these were detected by PCR-DGGE using a universal primer set. Monitoring revealed that the numbers of Escherichia coli/Shigella sonnei (97.9% similarity) were significantly higher in the diarrheal feces. Thus, PCR-DGGE is a useful tool for monitoring the main changes in bacterial floral diversity occurring in the gastrointestinal tracts of Thoroughbreds.

Original languageEnglish
Pages (from-to)14-19
Number of pages6
JournalJournal of Equine Veterinary Science
Volume27
Issue number1
DOIs
Publication statusPublished - Jan 1 2007
Externally publishedYes

Keywords

  • Denaturing gradient gel electrophoresis (DGGE)
  • Gastrointestinal tract
  • Thoroughbred foal

ASJC Scopus subject areas

  • Equine

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