Molecular markers for the self-compatible S4′-haplotype, a pollen-part Mutant in sweet cherry (Prunus avium L.)

K. Ikeda, A. Watari, Koichiro Ushijima, H. Yamane, N. R. Hauck, A. F. Iezzoni, R. Tao

Research output: Contribution to journalArticle

25 Citations (Scopus)

Abstract

S4′ is a pollen-part mutant in sweet cherry (Prunus avium L.) that is extensively used to develop self-compatible cultivars. The S 4′-haplotype is known to have a functional stylar component and a nonfunctional pollen component. The pollen component in sweet cherry necessary for the specificity of the pollen reaction is believed to be an S-haplotype specific F-box protein gene, called SFB. This study describes two molecular markers that distinguish between SFB4 and SFB 4′ by taking advantage of a four base pair deletion in the mutant allele. The resulting polymerase chain reaction (PCR) products can either be separated directly on a polyacrylamide gel or they can be subjected to restriction enzyme digestion and the different sized products can be visualized on an agarose gel. The latter technique utilizes restriction sites created in the PCR products from the SFB4′ allele, but not the SFB 4 allele. Because the primer sets created differential restriction sites, these primer sets were termed dCAPS (derived cleaved amplified polymorphism sequence) markers. These molecular assays can be used to verify self-compatibility conferred by the S4′-haplotype.

Original languageEnglish
Pages (from-to)724-728
Number of pages5
JournalJournal of the American Society for Horticultural Science
Volume129
Issue number5
Publication statusPublished - Sep 2004
Externally publishedYes

Fingerprint

Prunus avium
Pollen
Haplotypes
haplotypes
pollen
mutants
genetic markers
Alleles
alleles
polymerase chain reaction
F-Box Proteins
gels
F-box proteins
Polymerase Chain Reaction
polyacrylamide
Base Pairing
Sepharose
agarose
Digestion
Gels

Keywords

  • F-box protein
  • Self-incompatibility
  • SFB

ASJC Scopus subject areas

  • Horticulture
  • Plant Science

Cite this

Molecular markers for the self-compatible S4′-haplotype, a pollen-part Mutant in sweet cherry (Prunus avium L.). / Ikeda, K.; Watari, A.; Ushijima, Koichiro; Yamane, H.; Hauck, N. R.; Iezzoni, A. F.; Tao, R.

In: Journal of the American Society for Horticultural Science, Vol. 129, No. 5, 09.2004, p. 724-728.

Research output: Contribution to journalArticle

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