Molecular mapping of the Oregon Wolfe Barleys: A phenotypically polymorphic doubled-haploid population

J. M. Costa, A. Corey, P. M. Hayes, C. Jobet, A. Kleinhofs, A. Kopisch-Obusch, S. F. Kramer, D. Kudrna, M. Li, O. Riera-Lizarazu, Kazuhiro Sato, P. Szucs, T. Toojinda, M. I. Vales, R. I. Wolfe

Research output: Contribution to journalArticle

137 Citations (Scopus)

Abstract

A phenotypically polymorphic barley (Hordeum vulgare L.) mapping population was developed using morphological marker stocks as parents. Ninety-four doubled-haploid lines were derived for genetic mapping from an F1 using the Hordeum bulbosum system. A linkage map was constructed using 12 morphological markers, 87 restriction fragment length polymorphism (RFLP), five random amplified polymorphic DNA (RAPD), one sequence-tagged site (STS), one intron fragment length polymorphism (IFLP), 33 simple sequence repeat (SSR), and 586 amplified fragment length polymorphism (AFLP) markers. The genetic map spanned 1,387 cM with an average density of one marker every 1.9 cM. AFLP markers tended to cluster on centromeric regions and were more abundant on chromosome 1 (7H). RAPD markers showed a high level of segregation distortion, 54% compared with the 26% observed for AFLP markers, 27% for SSR markers, and 18% for RFLP markers. Three major regions of segregation distortion, based on RFLP and morphological markers, were located on chromosomes 2 (2H), 3 (3H), and 7 (5H). Segregation distortion may indicate that preferential gametic selection occurred during the development of the doubled-haploid lines. This may be due to the extreme phenotypes determined by alleles at morphological trait loci of the dominant and recessive parental stocks. Several molecular markers were found to be closely linked to morphological loci. The linkage map reported herein will be useful in integrating data on quantitative traits with morphological variants and should aid in map-based cloning of genes controlling morphological traits.

Original languageEnglish
Pages (from-to)415-424
Number of pages10
JournalTheoretical And Applied Genetics
Volume103
Issue number2-3
DOIs
Publication statusPublished - 2001

Fingerprint

segregation distortion
Haploidy
doubled haploids
Hordeum
Restriction Fragment Length Polymorphisms
amplified fragment length polymorphism
chromosome mapping
random amplified polymorphic DNA technique
restriction fragment length polymorphism
barley
Microsatellite Repeats
Hordeum bulbosum
Sequence Tagged Sites
microsatellite repeats
Population
chromosomes
loci
genetic markers
Chromosomes, Human, Pair 2
Chromosomes, Human, Pair 1

Keywords

  • Barley
  • Genetic map
  • Molecular markers
  • Morphological markers
  • Segregation distortion

ASJC Scopus subject areas

  • Agronomy and Crop Science
  • Horticulture
  • Plant Science
  • Genetics
  • Genetics(clinical)

Cite this

Costa, J. M., Corey, A., Hayes, P. M., Jobet, C., Kleinhofs, A., Kopisch-Obusch, A., ... Wolfe, R. I. (2001). Molecular mapping of the Oregon Wolfe Barleys: A phenotypically polymorphic doubled-haploid population. Theoretical And Applied Genetics, 103(2-3), 415-424. https://doi.org/10.1007/s001220100622

Molecular mapping of the Oregon Wolfe Barleys : A phenotypically polymorphic doubled-haploid population. / Costa, J. M.; Corey, A.; Hayes, P. M.; Jobet, C.; Kleinhofs, A.; Kopisch-Obusch, A.; Kramer, S. F.; Kudrna, D.; Li, M.; Riera-Lizarazu, O.; Sato, Kazuhiro; Szucs, P.; Toojinda, T.; Vales, M. I.; Wolfe, R. I.

In: Theoretical And Applied Genetics, Vol. 103, No. 2-3, 2001, p. 415-424.

Research output: Contribution to journalArticle

Costa, JM, Corey, A, Hayes, PM, Jobet, C, Kleinhofs, A, Kopisch-Obusch, A, Kramer, SF, Kudrna, D, Li, M, Riera-Lizarazu, O, Sato, K, Szucs, P, Toojinda, T, Vales, MI & Wolfe, RI 2001, 'Molecular mapping of the Oregon Wolfe Barleys: A phenotypically polymorphic doubled-haploid population', Theoretical And Applied Genetics, vol. 103, no. 2-3, pp. 415-424. https://doi.org/10.1007/s001220100622
Costa, J. M. ; Corey, A. ; Hayes, P. M. ; Jobet, C. ; Kleinhofs, A. ; Kopisch-Obusch, A. ; Kramer, S. F. ; Kudrna, D. ; Li, M. ; Riera-Lizarazu, O. ; Sato, Kazuhiro ; Szucs, P. ; Toojinda, T. ; Vales, M. I. ; Wolfe, R. I. / Molecular mapping of the Oregon Wolfe Barleys : A phenotypically polymorphic doubled-haploid population. In: Theoretical And Applied Genetics. 2001 ; Vol. 103, No. 2-3. pp. 415-424.
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AU - Jobet, C.

AU - Kleinhofs, A.

AU - Kopisch-Obusch, A.

AU - Kramer, S. F.

AU - Kudrna, D.

AU - Li, M.

AU - Riera-Lizarazu, O.

AU - Sato, Kazuhiro

AU - Szucs, P.

AU - Toojinda, T.

AU - Vales, M. I.

AU - Wolfe, R. I.

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