Abstract
Molybdenum cofactor (Moco) is essential for nitrate reductase (NR), xanthine dehydrogenase (XDH), and aldehyde oxidase to perform their catalytic functions in plants. Moco biosynthesis is a complex process involving many genes. Little is known about the genetics and molecular aspects of Moco biosynthesis in plants and other eukaryotes. In rice, we previously isolated a Moco mutant C25 with a mutation in the CNX2 gene from a mutagenized indica cultivar IR30 and characterized its biochemical properties. This mutant was crossed with a japonica cultivar, Norin 8, to investigate the linkage of cnx2 to restriction fragment length polymorphism (RFLP) and cleaved amplified polymorphic sequence (CAPS) markers. Chlorate resistance was used to trace the cnx2 mutation because of its cosegregation with the loss of NR and XDH activities observed earlier. RFLP and CAPS analyses show the location of the cnx2 locus on the long arm of chromosome 4. It is mapped between RFLP markers C513 and C377 with a distance of 9.5 and 13.1 cM, respectively. It is also linked with CAPS marker RA0738 at a distance of 30.3 cM.
| Original language | English |
|---|---|
| Pages (from-to) | 540-544 |
| Number of pages | 5 |
| Journal | Theoretical And Applied Genetics |
| Volume | 102 |
| Issue number | 4 |
| DOIs | |
| Publication status | Published - 2001 |
| Externally published | Yes |
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Keywords
- Cleaved amplified polymorphic sequences (CAPS)
- Molybdenum cofactor (Moco)
- Nitrate reductase (NR)
- RFLP
- Rice (Oryza sativa L.)
ASJC Scopus subject areas
- Agronomy and Crop Science
- Horticulture
- Plant Science
- Genetics
- Genetics(clinical)
Cite this
Molecular mapping of the cnx2 locus involved in molybdenum cofactor biosynthesis in rice (Oryza sativa L.). / Barlaan, E. A.; Sato, H.; Mushika, J.; Taketa, Shin; Ichii, M.
In: Theoretical And Applied Genetics, Vol. 102, No. 4, 2001, p. 540-544.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Molecular mapping of the cnx2 locus involved in molybdenum cofactor biosynthesis in rice (Oryza sativa L.)
AU - Barlaan, E. A.
AU - Sato, H.
AU - Mushika, J.
AU - Taketa, Shin
AU - Ichii, M.
PY - 2001
Y1 - 2001
N2 - Molybdenum cofactor (Moco) is essential for nitrate reductase (NR), xanthine dehydrogenase (XDH), and aldehyde oxidase to perform their catalytic functions in plants. Moco biosynthesis is a complex process involving many genes. Little is known about the genetics and molecular aspects of Moco biosynthesis in plants and other eukaryotes. In rice, we previously isolated a Moco mutant C25 with a mutation in the CNX2 gene from a mutagenized indica cultivar IR30 and characterized its biochemical properties. This mutant was crossed with a japonica cultivar, Norin 8, to investigate the linkage of cnx2 to restriction fragment length polymorphism (RFLP) and cleaved amplified polymorphic sequence (CAPS) markers. Chlorate resistance was used to trace the cnx2 mutation because of its cosegregation with the loss of NR and XDH activities observed earlier. RFLP and CAPS analyses show the location of the cnx2 locus on the long arm of chromosome 4. It is mapped between RFLP markers C513 and C377 with a distance of 9.5 and 13.1 cM, respectively. It is also linked with CAPS marker RA0738 at a distance of 30.3 cM.
AB - Molybdenum cofactor (Moco) is essential for nitrate reductase (NR), xanthine dehydrogenase (XDH), and aldehyde oxidase to perform their catalytic functions in plants. Moco biosynthesis is a complex process involving many genes. Little is known about the genetics and molecular aspects of Moco biosynthesis in plants and other eukaryotes. In rice, we previously isolated a Moco mutant C25 with a mutation in the CNX2 gene from a mutagenized indica cultivar IR30 and characterized its biochemical properties. This mutant was crossed with a japonica cultivar, Norin 8, to investigate the linkage of cnx2 to restriction fragment length polymorphism (RFLP) and cleaved amplified polymorphic sequence (CAPS) markers. Chlorate resistance was used to trace the cnx2 mutation because of its cosegregation with the loss of NR and XDH activities observed earlier. RFLP and CAPS analyses show the location of the cnx2 locus on the long arm of chromosome 4. It is mapped between RFLP markers C513 and C377 with a distance of 9.5 and 13.1 cM, respectively. It is also linked with CAPS marker RA0738 at a distance of 30.3 cM.
KW - Cleaved amplified polymorphic sequences (CAPS)
KW - Molybdenum cofactor (Moco)
KW - Nitrate reductase (NR)
KW - RFLP
KW - Rice (Oryza sativa L.)
UR - http://www.scopus.com/inward/record.url?scp=0035084823&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0035084823&partnerID=8YFLogxK
U2 - 10.1007/s001220051679
DO - 10.1007/s001220051679
M3 - Article
AN - SCOPUS:0035084823
VL - 102
SP - 540
EP - 544
JO - Theoretical And Applied Genetics
JF - Theoretical And Applied Genetics
SN - 0040-5752
IS - 4
ER -