Molecular mapping of the cnx2 locus involved in molybdenum cofactor biosynthesis in rice (Oryza sativa L.)

E. A. Barlaan, H. Sato, J. Mushika, Shin Taketa, M. Ichii

Research output: Contribution to journalArticle

Abstract

Molybdenum cofactor (Moco) is essential for nitrate reductase (NR), xanthine dehydrogenase (XDH), and aldehyde oxidase to perform their catalytic functions in plants. Moco biosynthesis is a complex process involving many genes. Little is known about the genetics and molecular aspects of Moco biosynthesis in plants and other eukaryotes. In rice, we previously isolated a Moco mutant C25 with a mutation in the CNX2 gene from a mutagenized indica cultivar IR30 and characterized its biochemical properties. This mutant was crossed with a japonica cultivar, Norin 8, to investigate the linkage of cnx2 to restriction fragment length polymorphism (RFLP) and cleaved amplified polymorphic sequence (CAPS) markers. Chlorate resistance was used to trace the cnx2 mutation because of its cosegregation with the loss of NR and XDH activities observed earlier. RFLP and CAPS analyses show the location of the cnx2 locus on the long arm of chromosome 4. It is mapped between RFLP markers C513 and C377 with a distance of 9.5 and 13.1 cM, respectively. It is also linked with CAPS marker RA0738 at a distance of 30.3 cM.

Original languageEnglish
Pages (from-to)540-544
Number of pages5
JournalTheoretical And Applied Genetics
Volume102
Issue number4
DOIs
Publication statusPublished - 2001
Externally publishedYes

Fingerprint

molybdenum
chromosome mapping
Oryza sativa
biosynthesis
Restriction Fragment Length Polymorphisms
Xanthine Dehydrogenase
xanthine dehydrogenase
Nitrate Reductase
rice
restriction fragment length polymorphism
loci
nitrate reductase
Aldehyde Oxidase
Chlorates
chlorates
mutation
mutants
Mutation
Chromosomes, Human, Pair 4
Xanthine Oxidase

Keywords

  • Cleaved amplified polymorphic sequences (CAPS)
  • Molybdenum cofactor (Moco)
  • Nitrate reductase (NR)
  • RFLP
  • Rice (Oryza sativa L.)

ASJC Scopus subject areas

  • Agronomy and Crop Science
  • Horticulture
  • Plant Science
  • Genetics
  • Genetics(clinical)

Cite this

Molecular mapping of the cnx2 locus involved in molybdenum cofactor biosynthesis in rice (Oryza sativa L.). / Barlaan, E. A.; Sato, H.; Mushika, J.; Taketa, Shin; Ichii, M.

In: Theoretical And Applied Genetics, Vol. 102, No. 4, 2001, p. 540-544.

Research output: Contribution to journalArticle

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