The Rfm1a gene restores the fertility of msm1 cytoplasmic male-sterile lines in barley. We identified three RAPD markers linked to the Rfm1 locus (CMNB-07/800, OPI-18/900, and OPT-02/700) using isogenic lines and segregating BC1F1 and F2 populations. Using a previously developed linkage map of barley, we located CMNB-07/800 and OPT-02/700 beside MWG2218 on chromosome 6HS. The linkage between MWG2218 and the Rfm1 locus was demonstrated using the segregating BC1F1 and F2 populations. To confirm the chromosomal locations of these markers, we converted them to STSs and tested against two sets of wheat-barley chromosome addition lines. These STS markers, CMNB-07/800, OPT02/700, and MWG2218, were amplified only in the addition lines possessing the chromosome 6H, thereby providing additional evidence the Rfm1 locus is located on chromosome 6H. Homoeologous relationships among fertility restoration genes in Triticeae are discussed.
- Cytoplasmic male sterility (CMS)
- Isogenic lines
- Randomly amplified polymorphic DNA (RAPD)
- Restorer gene
- Sequence tagged site (STS)
ASJC Scopus subject areas
- Agronomy and Crop Science