Molecular identification of a eukaryotic, stretch-activated nonselective cation channel

Makoto Kanzaki, Masahiro Nagasawa, Itaru Kojima, Chikara Sato, Keiji Naruse, Masahiro Sokabe, Hidetoshi Iida

Research output: Contribution to journalArticle

162 Citations (Scopus)

Abstract

Calcium-permeable, stretch-activated nonselective cation ($A Cat) channels mediate cellular responses to mechanical stimuli. However, genes encoding such channels have not been identified in eukaryotes. The yeast MID1 gene product (Mid1) is required for calcium influx in the yeast Saccharomyces cerevisiae. Functional expression of Mid1 in Chinese hamster ovary cells conferred sensitivity to mechanical stress that resulted in increases in both calcium conductance and the concentration of cytosolic free calcium. These increases were dependent on the presence of extracellular calcium and were reduced by gadolinium, a blocker of SA Cat channels. Single-channel analyses with cell-attached patches revealed that Mid1 acts as a calcium-permeable, cation-selective stretch-activated channel with a conductance of 32 picosiemens at 150 millimolar cesium chloride in the pipette. Thus, Mid1 appears to be a eukaryotic, SA Cat channel.

Original languageEnglish
Pages (from-to)882-886
Number of pages5
JournalScience
Volume285
Issue number5429
DOIs
Publication statusPublished - Aug 6 1999
Externally publishedYes

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Cations
Calcium
Cats
Yeasts
Mechanical Stress
Gadolinium
Cricetulus
Eukaryota
Genes
Saccharomyces cerevisiae
Ovary

ASJC Scopus subject areas

  • General

Cite this

Kanzaki, M., Nagasawa, M., Kojima, I., Sato, C., Naruse, K., Sokabe, M., & Iida, H. (1999). Molecular identification of a eukaryotic, stretch-activated nonselective cation channel. Science, 285(5429), 882-886. https://doi.org/10.1126/science.285.5429.882

Molecular identification of a eukaryotic, stretch-activated nonselective cation channel. / Kanzaki, Makoto; Nagasawa, Masahiro; Kojima, Itaru; Sato, Chikara; Naruse, Keiji; Sokabe, Masahiro; Iida, Hidetoshi.

In: Science, Vol. 285, No. 5429, 06.08.1999, p. 882-886.

Research output: Contribution to journalArticle

Kanzaki, M, Nagasawa, M, Kojima, I, Sato, C, Naruse, K, Sokabe, M & Iida, H 1999, 'Molecular identification of a eukaryotic, stretch-activated nonselective cation channel', Science, vol. 285, no. 5429, pp. 882-886. https://doi.org/10.1126/science.285.5429.882
Kanzaki, Makoto ; Nagasawa, Masahiro ; Kojima, Itaru ; Sato, Chikara ; Naruse, Keiji ; Sokabe, Masahiro ; Iida, Hidetoshi. / Molecular identification of a eukaryotic, stretch-activated nonselective cation channel. In: Science. 1999 ; Vol. 285, No. 5429. pp. 882-886.
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