Molecular identification of 1-Cys peroxiredoxin and anthocyanidin/flavonol 3-O-galactosyltransferase from proanthocyanidin-rich young fruits of persimmon (Diospyros kaki Thunb.)

Ayako Ikegami, Takashi Akagi, Daniel Potter, Masahiko Yamada, Akihiko Sato, Keizo Yonemori, Akira Kitajima, Kentaro Inoue

Research output: Contribution to journalArticle

26 Citations (Scopus)

Abstract

Fruits of persimmon (Diospyros kaki Thunb.) accumulate large amounts of proanthocyanidins (PAs) in the early stages of development. Astringent (A)-type fruits remain rich in soluble PAs even after they reach full-mature stage, whereas non-astringent (NA)-type fruits lose these compounds before full maturation. As a first step to elucidate the mechanism of PA accumulation in this non-model species, we used suppression subtractive hybridization to identify transcripts accumulating differently in young fruits of A- and NA-type. Interestingly, only a few clones involved in PA biosynthesis were identified in A-NA libraries. Represented by multiple clones were those encoding a novel 1-Cys peroxiredoxin and a new member of family 1 glycosyltransferases. Quantitative RT-PCR analyses confirmed correlation of the amount of PAs and accumulation of transcripts encoding these proteins in young persimmon fruits. Furthermore, the new family 1 glycosyltransferase was produced in Escherichia coli and shown to efficiently catalyze galactosylation at 3-hydroxyl groups of several anthocyanidins and flavonols. These findings suggest a complex mechanism of PA accumulation in persimmon fruits.

Original languageEnglish
Pages (from-to)841-855
Number of pages15
JournalPlanta
Volume230
Issue number4
DOIs
Publication statusPublished - Sep 1 2009
Externally publishedYes

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Keywords

  • 1-Cys peroxiredoxin
  • Diospyros kaki
  • Flavonoid 3-O-galactosyltransferase
  • Fruits
  • Persimmon
  • Proanthocyanidin

ASJC Scopus subject areas

  • Genetics
  • Plant Science

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