Molecular evidence for biochemical diversification of phenolamide biosynthesis in rice plants

Kimiaki Tanabe, Yuko Hojo, Tomonori Shinya, Ivan Galis

    Research output: Contribution to journalArticlepeer-review

    17 Citations (Scopus)

    Abstract

    Two phenolamides (PAs), p-coumaroylputrescine and feruloylputrescine strongly accumulate in rice (Oryza sativa cv. Nipponbare) leaves subjected to attack of chewing and sucking herbivores. Here we identified and characterized in vitro three novel rice genes that mediated coumaroyl-CoA/feruloyl-CoA conjugation to polyamines, putrescine and agmatine. Interestingly, two genes were highly specific for their polyamine substrates, encoding putrescine N-hydroxycinnamoyltransferase and agmatine N-hydroxycinnamoyltransferase, while the third enzyme could use both polyamines and it was therefore annotated as putrescine/agmatine N-hydroxycinnamoyltransferase. All genes were preferentially expressed in rice roots and developing flowers, and in addition, the putrescine/agmatine N-hydroxycinnamoyltransferase transcripts were strongly induced by wounding in the young rice leaves. Because the wound response of this gene was only partially suppressed in the jasmonoyl-L-isoleucine deficient plants (Osjar1), it suggests that its upregulation (as well as inducible PAs in rice) may be largely independent of jasmonoyl-L-isoleucine signaling pathway. The finding of three closely related genes with a similar and/or overlapping activity in PA biosynthesis provides another striking example of rapid diversification of plant metabolism in response to environmental stresses in nature.

    Original languageEnglish
    Pages (from-to)903-913
    Number of pages11
    JournalJournal of Integrative Plant Biology
    Volume58
    Issue number11
    DOIs
    Publication statusPublished - Nov 1 2016

    Keywords

    • Acyltransferase
    • defense
    • feruloylputrescine
    • p-coumaroylputrescine
    • phenolamides
    • rice (Oryza sativa)

    ASJC Scopus subject areas

    • Biochemistry
    • Biochemistry, Genetics and Molecular Biology(all)
    • Plant Science

    Fingerprint

    Dive into the research topics of 'Molecular evidence for biochemical diversification of phenolamide biosynthesis in rice plants'. Together they form a unique fingerprint.

    Cite this