Molecular epidemiology of paramyxoviruses in Frugivorous Eidolon helvum bats in Zambia

Walter Muleya, Michihito Sasaki, Yasuko Orba, Akihiro Ishii, Yuka Thomas, Emiko Nakagawa, Hirohito Ogawa, Bernard Hang'ombe, Boniface Namangala, Aaron Mweene, Ayato Takada, Takashi Kimura, Hirofumi Sawa

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

In this study, we describe the detection of novel paramyxoviruses from the Eidolon helvum species of fruit bats. We extracted RNA from 312 spleen samples from bats captured in Zambia over a period of 4 years (2008-2011). Semi-nested RT-PCR detected a total of 25 (8%) positive samples for paramyxoviruses which were then directly sequenced and analyzed using phylogenetic analysis. Among the positive samples, seven novel paramyxoviruses were detected. Five viruses were closely related to the genus Henipavirus, while two viruses were related to the unclassified Bat paramyxoviruses from Ghana and Congo Brazzaville. Our study identified novel Henipavirus-related and unrelated viruses using RT-PCR in fruit bats from Kansaka National Park and indicated the presence of similar Bat paramyxoviruses originating from wide geographic areas, suggesting the ability of bats to harbor and transmit viruses. The presence of these viruses in fruit bats might pose a public health risk.

Original languageEnglish
Pages (from-to)611-614
Number of pages4
JournalJournal of Veterinary Medical Science
Volume76
Issue number4
DOIs
Publication statusPublished - 2014
Externally publishedYes

Fingerprint

Respirovirus
Zambia
molecular epidemiology
Molecular Epidemiology
Chiroptera
Henipavirus
Viruses
viruses
Fruit
fruits
reverse transcriptase polymerase chain reaction
Congo
Polymerase Chain Reaction
Ghana
Republic of the Congo
sampling
national parks
public health
spleen
Spleen

Keywords

  • Bat viruses
  • Epidemiology
  • Paramyxoviruses
  • Virus
  • Zambia

ASJC Scopus subject areas

  • veterinary(all)
  • Medicine(all)

Cite this

Muleya, W., Sasaki, M., Orba, Y., Ishii, A., Thomas, Y., Nakagawa, E., ... Sawa, H. (2014). Molecular epidemiology of paramyxoviruses in Frugivorous Eidolon helvum bats in Zambia. Journal of Veterinary Medical Science, 76(4), 611-614. https://doi.org/10.1292/jvms.13-0518

Molecular epidemiology of paramyxoviruses in Frugivorous Eidolon helvum bats in Zambia. / Muleya, Walter; Sasaki, Michihito; Orba, Yasuko; Ishii, Akihiro; Thomas, Yuka; Nakagawa, Emiko; Ogawa, Hirohito; Hang'ombe, Bernard; Namangala, Boniface; Mweene, Aaron; Takada, Ayato; Kimura, Takashi; Sawa, Hirofumi.

In: Journal of Veterinary Medical Science, Vol. 76, No. 4, 2014, p. 611-614.

Research output: Contribution to journalArticle

Muleya, W, Sasaki, M, Orba, Y, Ishii, A, Thomas, Y, Nakagawa, E, Ogawa, H, Hang'ombe, B, Namangala, B, Mweene, A, Takada, A, Kimura, T & Sawa, H 2014, 'Molecular epidemiology of paramyxoviruses in Frugivorous Eidolon helvum bats in Zambia', Journal of Veterinary Medical Science, vol. 76, no. 4, pp. 611-614. https://doi.org/10.1292/jvms.13-0518
Muleya, Walter ; Sasaki, Michihito ; Orba, Yasuko ; Ishii, Akihiro ; Thomas, Yuka ; Nakagawa, Emiko ; Ogawa, Hirohito ; Hang'ombe, Bernard ; Namangala, Boniface ; Mweene, Aaron ; Takada, Ayato ; Kimura, Takashi ; Sawa, Hirofumi. / Molecular epidemiology of paramyxoviruses in Frugivorous Eidolon helvum bats in Zambia. In: Journal of Veterinary Medical Science. 2014 ; Vol. 76, No. 4. pp. 611-614.
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