Molecular epidemiological studies of Vibrio cholerae in Bengal region

Sumio Shinoda, Tomoko Nakagawa, Nobuyuki Hirakawa, Shin-ichi Miyoshi, Eiji Arakawa, Thandavarayan Ramamurthy, B. Dutta, Shah M. Faruque, Gopinath Balakrish Nair

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

Vibrio cholerae isolates from environmental and clinical origins in the Bengal region in which epidemics of cholera break out periodically were analyzed with particular emphasis on the molecular epidemiological features. The presence of the virulence genes (ctxA, tcpA and toxR) in the isolates was analyzed by the PCR (polymerase chain reaction) method. PFGE (pulsed-field gel electrophoresis) was performed to determine the clonal relationships between the clinical and environmental strains. Antibiograms and O serovars of the isolates were also examined. O1 and O139 strains from both clinical and environmental sources were all positive for the three virulence genes while non-O1 /non-O139 strains from both sources were all negative for ctxA and tcpA but positive for toxR. PFGE patterns of recent isolates of O1 and O139 were similar in each serovar regardless of origin, suggesting a clonal relationship between the clinical and environmental strains, although comparison with past isolates or isolates from different geographical area showed some differences.

Original languageEnglish
Pages (from-to)1-8
Number of pages8
JournalBiocontrol Science
Volume13
Issue number1
Publication statusPublished - Mar 2008

Fingerprint

Vibrio cholerae
Pulsed Field Gel Electrophoresis
Virulence
Epidemiologic Studies
Cholera
Microbial Sensitivity Tests
Electrophoresis
Genes
Gels
Polymerase Chain Reaction
Polymerase chain reaction
Serogroup

Keywords

  • Bengal region
  • Molecular epidemiology
  • Pulsed-field gel electrophoresis
  • Vibrio cholerae

ASJC Scopus subject areas

  • Environmental Engineering

Cite this

Shinoda, S., Nakagawa, T., Hirakawa, N., Miyoshi, S., Arakawa, E., Ramamurthy, T., ... Nair, G. B. (2008). Molecular epidemiological studies of Vibrio cholerae in Bengal region. Biocontrol Science, 13(1), 1-8.

Molecular epidemiological studies of Vibrio cholerae in Bengal region. / Shinoda, Sumio; Nakagawa, Tomoko; Hirakawa, Nobuyuki; Miyoshi, Shin-ichi; Arakawa, Eiji; Ramamurthy, Thandavarayan; Dutta, B.; Faruque, Shah M.; Nair, Gopinath Balakrish.

In: Biocontrol Science, Vol. 13, No. 1, 03.2008, p. 1-8.

Research output: Contribution to journalArticle

Shinoda, S, Nakagawa, T, Hirakawa, N, Miyoshi, S, Arakawa, E, Ramamurthy, T, Dutta, B, Faruque, SM & Nair, GB 2008, 'Molecular epidemiological studies of Vibrio cholerae in Bengal region', Biocontrol Science, vol. 13, no. 1, pp. 1-8.
Shinoda S, Nakagawa T, Hirakawa N, Miyoshi S, Arakawa E, Ramamurthy T et al. Molecular epidemiological studies of Vibrio cholerae in Bengal region. Biocontrol Science. 2008 Mar;13(1):1-8.
Shinoda, Sumio ; Nakagawa, Tomoko ; Hirakawa, Nobuyuki ; Miyoshi, Shin-ichi ; Arakawa, Eiji ; Ramamurthy, Thandavarayan ; Dutta, B. ; Faruque, Shah M. ; Nair, Gopinath Balakrish. / Molecular epidemiological studies of Vibrio cholerae in Bengal region. In: Biocontrol Science. 2008 ; Vol. 13, No. 1. pp. 1-8.
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