Molecular epidemiological studies of Staphylococcus aureus in urinary tract infection

Motoo Araki, R. Kariyama, K. Monden, M. Tsugawa, H. Kumon

Research output: Contribution to journalArticle

16 Citations (Scopus)

Abstract

In recent years, the increasing incidence of urinary tract infection (UTIs) caused by Staphylococcus aureus has been noted at the urology ward, Okayama University Hospital. We investigated the molecular epidemiological characteristics of 139 UTI isolates, including 45 methicillin-sensitive S. aureus (MSSA) and 94 methicillin-resistant S. aureus (MRSA), collected over a 10-year period from 1990 to 1999. The antibiotic resistance genes (mecA, aph(3′)-III, aac(6′)-aph(2″), ant(4′)-I) and the toxin genes (tst, sea, seb, and sec) were detected by using multiplex polymerase chain reaction (PCR). Since 1996, the prevalence of the ant(4′)-I, tst and sec genes has increased markedly in coagulase type II S. aureus possessing the mecA gene (MRSA). The presence of toxin genes in MRSA was higher than that in MSSA; 66.0% and 26.7% for tst, 7.4% and 4.4% for sea, 24.5% and 8.9% for seb, and 66.0% and 28.9% for sec, respectively. In the review of medical records, it was found that febrile episodes occurred in 12 of 72 patients with monomicrobial UTI caused by S. aureus. For the febrile patients, S. aureus isolates with both the tst and sec genes were found significantly more often (11 of 12; 91.7%) than those without the tst and sec genes (P = 0.0484). Molecular typing of MRSA isolates, by using random amplified polymorphic DNA analysis and pulsed-field gel electrophoresis analysis, revealed no apparent clonality of these isolates over the 10 years, suggesting that most of the recent MRSA infections are not due to cross-infection in the urology ward.

Original languageEnglish
Pages (from-to)168-174
Number of pages7
JournalJournal of Infection and Chemotherapy
Volume8
Issue number2
DOIs
Publication statusPublished - 2002

Fingerprint

Urinary Tract Infections
Staphylococcus aureus
Epidemiologic Studies
Methicillin-Resistant Staphylococcus aureus
Genes
Methicillin
Ants
Urology
Oceans and Seas
Fever
Molecular Typing
Coagulase
Pulsed Field Gel Electrophoresis
Multiplex Polymerase Chain Reaction
Microbial Drug Resistance
Cross Infection
Medical Records
DNA
Incidence
Infection

Keywords

  • Aminoglycoside resistance genes
  • Enterotoxin C gene
  • Molecular epidemiology
  • Staphylococcus aureus
  • Toxic shock syndrome toxin-1 gene
  • Urinary tract infection

ASJC Scopus subject areas

  • Microbiology (medical)

Cite this

Molecular epidemiological studies of Staphylococcus aureus in urinary tract infection. / Araki, Motoo; Kariyama, R.; Monden, K.; Tsugawa, M.; Kumon, H.

In: Journal of Infection and Chemotherapy, Vol. 8, No. 2, 2002, p. 168-174.

Research output: Contribution to journalArticle

Araki, Motoo ; Kariyama, R. ; Monden, K. ; Tsugawa, M. ; Kumon, H. / Molecular epidemiological studies of Staphylococcus aureus in urinary tract infection. In: Journal of Infection and Chemotherapy. 2002 ; Vol. 8, No. 2. pp. 168-174.
@article{c3f2e23e4b8e43619f11fd50699df01d,
title = "Molecular epidemiological studies of Staphylococcus aureus in urinary tract infection",
abstract = "In recent years, the increasing incidence of urinary tract infection (UTIs) caused by Staphylococcus aureus has been noted at the urology ward, Okayama University Hospital. We investigated the molecular epidemiological characteristics of 139 UTI isolates, including 45 methicillin-sensitive S. aureus (MSSA) and 94 methicillin-resistant S. aureus (MRSA), collected over a 10-year period from 1990 to 1999. The antibiotic resistance genes (mecA, aph(3′)-III, aac(6′)-aph(2″), ant(4′)-I) and the toxin genes (tst, sea, seb, and sec) were detected by using multiplex polymerase chain reaction (PCR). Since 1996, the prevalence of the ant(4′)-I, tst and sec genes has increased markedly in coagulase type II S. aureus possessing the mecA gene (MRSA). The presence of toxin genes in MRSA was higher than that in MSSA; 66.0{\%} and 26.7{\%} for tst, 7.4{\%} and 4.4{\%} for sea, 24.5{\%} and 8.9{\%} for seb, and 66.0{\%} and 28.9{\%} for sec, respectively. In the review of medical records, it was found that febrile episodes occurred in 12 of 72 patients with monomicrobial UTI caused by S. aureus. For the febrile patients, S. aureus isolates with both the tst and sec genes were found significantly more often (11 of 12; 91.7{\%}) than those without the tst and sec genes (P = 0.0484). Molecular typing of MRSA isolates, by using random amplified polymorphic DNA analysis and pulsed-field gel electrophoresis analysis, revealed no apparent clonality of these isolates over the 10 years, suggesting that most of the recent MRSA infections are not due to cross-infection in the urology ward.",
keywords = "Aminoglycoside resistance genes, Enterotoxin C gene, Molecular epidemiology, Staphylococcus aureus, Toxic shock syndrome toxin-1 gene, Urinary tract infection",
author = "Motoo Araki and R. Kariyama and K. Monden and M. Tsugawa and H. Kumon",
year = "2002",
doi = "10.1007/s101560200029",
language = "English",
volume = "8",
pages = "168--174",
journal = "Journal of Infection and Chemotherapy",
issn = "1341-321X",
publisher = "Elsevier BV",
number = "2",

}

TY - JOUR

T1 - Molecular epidemiological studies of Staphylococcus aureus in urinary tract infection

AU - Araki, Motoo

AU - Kariyama, R.

AU - Monden, K.

AU - Tsugawa, M.

AU - Kumon, H.

PY - 2002

Y1 - 2002

N2 - In recent years, the increasing incidence of urinary tract infection (UTIs) caused by Staphylococcus aureus has been noted at the urology ward, Okayama University Hospital. We investigated the molecular epidemiological characteristics of 139 UTI isolates, including 45 methicillin-sensitive S. aureus (MSSA) and 94 methicillin-resistant S. aureus (MRSA), collected over a 10-year period from 1990 to 1999. The antibiotic resistance genes (mecA, aph(3′)-III, aac(6′)-aph(2″), ant(4′)-I) and the toxin genes (tst, sea, seb, and sec) were detected by using multiplex polymerase chain reaction (PCR). Since 1996, the prevalence of the ant(4′)-I, tst and sec genes has increased markedly in coagulase type II S. aureus possessing the mecA gene (MRSA). The presence of toxin genes in MRSA was higher than that in MSSA; 66.0% and 26.7% for tst, 7.4% and 4.4% for sea, 24.5% and 8.9% for seb, and 66.0% and 28.9% for sec, respectively. In the review of medical records, it was found that febrile episodes occurred in 12 of 72 patients with monomicrobial UTI caused by S. aureus. For the febrile patients, S. aureus isolates with both the tst and sec genes were found significantly more often (11 of 12; 91.7%) than those without the tst and sec genes (P = 0.0484). Molecular typing of MRSA isolates, by using random amplified polymorphic DNA analysis and pulsed-field gel electrophoresis analysis, revealed no apparent clonality of these isolates over the 10 years, suggesting that most of the recent MRSA infections are not due to cross-infection in the urology ward.

AB - In recent years, the increasing incidence of urinary tract infection (UTIs) caused by Staphylococcus aureus has been noted at the urology ward, Okayama University Hospital. We investigated the molecular epidemiological characteristics of 139 UTI isolates, including 45 methicillin-sensitive S. aureus (MSSA) and 94 methicillin-resistant S. aureus (MRSA), collected over a 10-year period from 1990 to 1999. The antibiotic resistance genes (mecA, aph(3′)-III, aac(6′)-aph(2″), ant(4′)-I) and the toxin genes (tst, sea, seb, and sec) were detected by using multiplex polymerase chain reaction (PCR). Since 1996, the prevalence of the ant(4′)-I, tst and sec genes has increased markedly in coagulase type II S. aureus possessing the mecA gene (MRSA). The presence of toxin genes in MRSA was higher than that in MSSA; 66.0% and 26.7% for tst, 7.4% and 4.4% for sea, 24.5% and 8.9% for seb, and 66.0% and 28.9% for sec, respectively. In the review of medical records, it was found that febrile episodes occurred in 12 of 72 patients with monomicrobial UTI caused by S. aureus. For the febrile patients, S. aureus isolates with both the tst and sec genes were found significantly more often (11 of 12; 91.7%) than those without the tst and sec genes (P = 0.0484). Molecular typing of MRSA isolates, by using random amplified polymorphic DNA analysis and pulsed-field gel electrophoresis analysis, revealed no apparent clonality of these isolates over the 10 years, suggesting that most of the recent MRSA infections are not due to cross-infection in the urology ward.

KW - Aminoglycoside resistance genes

KW - Enterotoxin C gene

KW - Molecular epidemiology

KW - Staphylococcus aureus

KW - Toxic shock syndrome toxin-1 gene

KW - Urinary tract infection

UR - http://www.scopus.com/inward/record.url?scp=0036022477&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0036022477&partnerID=8YFLogxK

U2 - 10.1007/s101560200029

DO - 10.1007/s101560200029

M3 - Article

C2 - 12111571

AN - SCOPUS:0036022477

VL - 8

SP - 168

EP - 174

JO - Journal of Infection and Chemotherapy

JF - Journal of Infection and Chemotherapy

SN - 1341-321X

IS - 2

ER -