Molecular detection of metastatic retinoblastoma cells by reverse transcription polymerase reaction for interphotoreceptor retinoid-binding protein mRNA

A case report

Nobuko Yamashita, Ritsuo Nishiuchi, Megumi Oda, Yoshie Tomiyama, Naohiro Eguchi, Chie Endo, Akira Manki, Yoshiki Seino

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

BACKGROUND. In the current study, the authors report a 4 year old girl with disseminated retinoblastoma. To find sensitive and specific molecular markers for detection of retinoblastoma cells in blood and marrow, the authors evaluated three photoreceptor-associated gene transcripts by using reverse transcription polymerase chain reaction (RT-PCR). METHOD. Samples of bone marrow and blood were obtained from healthy donors and the patient. RT-PCR was performed to detect the cone α′-subunit of cGMP phosphodiesterase (cone α′-PDE), the rod β-subunit of cGMP (rod β-PDE), and the interphotoreceptor retinoid-binding protein (IRBP) gene transcript in RNA extracted from the samples. RESULTS. While no expression of rod β-PDE or IRBP was detected in any of the normal samples, expression of cone α′-PDE was detected in two out of seven normal marrow samples. Expression of rod β-PDE was not detected in the patient samples. Expression of IRBP was detected in the patient samples obtained from iliac bone marrow before intensive chemotherapy but not thereafter. CONCLUSION. RT-PCR for IRBP was a useful method for detecting metastatic retinoblastoma cells as well as for evaluating the therapeutic effects of treatment in this particular case.

Original languageEnglish
Pages (from-to)1568-1573
Number of pages6
JournalCancer
Volume91
Issue number8
DOIs
Publication statusPublished - Apr 15 2001

Fingerprint

Retinoblastoma
Reverse Transcription
Bone Marrow
Messenger RNA
Phosphoric Diester Hydrolases
Polymerase Chain Reaction
Therapeutic Uses
Genes
Blood Cells
Tissue Donors
RNA
Drug Therapy
interstitial retinol-binding protein
Therapeutics

Keywords

  • Hematopoietic stem cell transplantation
  • Polymerase chain reaction
  • Retinoblastoma
  • Retinol-binding proteins

ASJC Scopus subject areas

  • Cancer Research
  • Oncology

Cite this

Molecular detection of metastatic retinoblastoma cells by reverse transcription polymerase reaction for interphotoreceptor retinoid-binding protein mRNA : A case report. / Yamashita, Nobuko; Nishiuchi, Ritsuo; Oda, Megumi; Tomiyama, Yoshie; Eguchi, Naohiro; Endo, Chie; Manki, Akira; Seino, Yoshiki.

In: Cancer, Vol. 91, No. 8, 15.04.2001, p. 1568-1573.

Research output: Contribution to journalArticle

Yamashita, Nobuko ; Nishiuchi, Ritsuo ; Oda, Megumi ; Tomiyama, Yoshie ; Eguchi, Naohiro ; Endo, Chie ; Manki, Akira ; Seino, Yoshiki. / Molecular detection of metastatic retinoblastoma cells by reverse transcription polymerase reaction for interphotoreceptor retinoid-binding protein mRNA : A case report. In: Cancer. 2001 ; Vol. 91, No. 8. pp. 1568-1573.
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