Molecular definition of human β2-glycoprotein (α2-GPI) by cDNA cloning and inter-species differences of β2-GPI in alternation of anticardiolipin binding

Matsuura Eiji, Makoto Igarashi, Yoshiko Igarashi, Hisato Nagae, Kenji Ichikawa, Tatsuji Yasuda, Takao Koike

    Research output: Contribution to journalArticlepeer-review

    78 Citations (Scopus)

    Abstract

    Human β2-glycoprotein I (β2-GPI) is involved in cardiolipin (CL) binding of anticardiolipin antibodies (aCL) in systemic lupus erythematosus (SLE). We examined the inter-species differences of β2-GPl in alternation of CL binding of aCL. β2-GPI preparations were obtained from human, bovine, and rat sera by sequential CL-polyacrylamide affinity, DEAE - cellulose, and anti-human IgG-conjugated Sepharose CL-4B column chromatography, and they had apparent molecular weights of 50, 53, and 55 kDa respectively. Human β2-GPI not only enhanced CL binding by aCL in SLE but also depressed it by those in syphilis. Either bovine and rat β2-GPI exerted no or quite small inhibition of the binding of syphilitic aCL compared with human β2-GPI whereas all three species of β2-GPI generated binding of aCL in SLE to a similar degree. Further, a complete cDNA clone, pβ2-GPI, was isolated from a human hepatoma cell line, HepG2, and its nucleotide sequence was analyzed. The sequences of bovine and rat counterpart molecules (β2-GPI) are highly homologous to that of the deduced sequence, and their corresponding regions are 84.0 and 82.5% identical to the complete domain and to the amino acid sequence 53-326 of human β2-GPI respectively. One of major differences appears at position 154 in human β2-GPI, and might be associated with the inhibitory effect on the binding of syphilitic aCL. The sequencing analysis of these β2-GPI proteins might provide leads to functional sites of domains which would be associated with such serological phenomena.

    Original languageEnglish
    Pages (from-to)1217-1221
    Number of pages5
    JournalInternational Immunology
    Volume3
    Issue number12
    DOIs
    Publication statusPublished - Dec 1991

    Keywords

    • Cardiolipin
    • Syphilis
    • Systemic lupus erythematosus
    • β2-glycoprotein I

    ASJC Scopus subject areas

    • Immunology and Allergy
    • Immunology

    Fingerprint

    Dive into the research topics of 'Molecular definition of human β<sub>2</sub>-glycoprotein (α<sub>2</sub>-GPI) by cDNA cloning and inter-species differences of β<sub>2</sub>-GPI in alternation of anticardiolipin binding'. Together they form a unique fingerprint.

    Cite this