Molecular cloning, sequencing, and gene expression analysis of tributyltin-binding protein type 1 in Japanese medaka fish, Oryzias latipes

Mohamed Nassef, Yoko Kato-Unoki, Tomohisa Furuta, Kei Nakayama, Hina Satone, Yohei Shimasaki, Tsuneo Honjo, Yuji Oshima

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

The full-length cDNA sequence of tributyltin-binding protein type1 in Japanese medaka (Oryzias latipes) (Olat.TBT-bp1) was determined by means of rapid amplification of cDNA ends (RACE) of liver tissue. Analysis of the structure of the gene encoding Olat.TBT-bp1 revealed that the exonintron organization of this gene corresponds to that of the genes encoding lipocalin superfamily proteins, suggesting that Olat.TBT-bp1 can be categorized as a member of the lipocalin superfamily, which may play an important role in transportation, detoxification, and excretion of xenobiotic compounds. Reverse transcription - PCR revealed that Olat.TBT-bp1 was expressed mainly in the liver, and upregulation of its expression was detected 1, 2, and 4 weeks post hatching. Relative expression of the Olat.TBT-bp1 gene was significantly downregulated, compared with that in the solvent control, by exposure to tributyltin at 0.01 mg/l or triclosan at 1.7 mg/l. Further studies on Olat.TBT-bp1 expression in conjunction with other biochemical and physiological toxicities in response to chemical exposures are needed to increase our understanding and information of TBT-bps mechanisms and as molecular biomarkers of chemical exposures. The role of Olat.TBT-bp1 in xenobiotic detoxification and/or excretion needs more investigations.

Original languageEnglish
Pages (from-to)281-285
Number of pages5
JournalZoological science
Volume28
Issue number4
DOIs
Publication statusPublished - Apr 1 2011

Keywords

  • cloning
  • detoxification
  • gene expression
  • lipocalin
  • transportation

ASJC Scopus subject areas

  • Animal Science and Zoology

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    Nassef, M., Kato-Unoki, Y., Furuta, T., Nakayama, K., Satone, H., Shimasaki, Y., Honjo, T., & Oshima, Y. (2011). Molecular cloning, sequencing, and gene expression analysis of tributyltin-binding protein type 1 in Japanese medaka fish, Oryzias latipes. Zoological science, 28(4), 281-285. https://doi.org/10.2108/zsj.28.281