Molecular cloning, sequencing, and expression of a cDNA encoding α-glucosidase from Mucor javanicus

Manabu Sugimoto, Yukio Suzuki

Research output: Contribution to journalArticle

18 Citations (Scopus)

Abstract

A cDNA encoding Mucor javanicus α-glucosidase was cloned and sequenced by the reverse transcription-polymerase chain reaction and rapid amplification of cDNA ends methods. The cDNA comprised 2,751 bp, and included an open reading frame which encodes a polypeptide of 864 amino acid residues with a molecular mass of 98,759 Da. The deduced amino acid sequence showed homology to fungal and mammalian α-glucosidases and related enzymes, and the sequence around the putative active site was well conserved among these enzymes. The cloned gene was expressed in Escherichia coli cells to produce the α-glucosidase, which hydrolyzes not only maltose, but also soluble starch.

Original languageEnglish
Pages (from-to)500-505
Number of pages6
JournalJournal of Biochemistry
Volume119
Issue number3
Publication statusPublished - Mar 1996

Fingerprint

Glucosidases
Mucor
Cloning
Molecular Cloning
Complementary DNA
Amino Acids
Amino Acid Sequence Homology
Maltose
Polymerase chain reaction
Molecular mass
Enzymes
Transcription
Starch
Escherichia coli
Open Reading Frames
Reverse Transcription
Amplification
Catalytic Domain
Genes
Polymerase Chain Reaction

Keywords

  • α-glucosidase
  • Gene expression
  • Mucor javanicus
  • Nucleotide sequence

ASJC Scopus subject areas

  • Biochemistry

Cite this

Molecular cloning, sequencing, and expression of a cDNA encoding α-glucosidase from Mucor javanicus. / Sugimoto, Manabu; Suzuki, Yukio.

In: Journal of Biochemistry, Vol. 119, No. 3, 03.1996, p. 500-505.

Research output: Contribution to journalArticle

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