Molecular cloning, sequencing, and expression of a cDNA encoding α-glucosidase from Mucor javanicus

Manabu Sugimoto, Yukio Suzuki

    Research output: Contribution to journalArticlepeer-review

    18 Citations (Scopus)

    Abstract

    A cDNA encoding Mucor javanicus α-glucosidase was cloned and sequenced by the reverse transcription-polymerase chain reaction and rapid amplification of cDNA ends methods. The cDNA comprised 2,751 bp, and included an open reading frame which encodes a polypeptide of 864 amino acid residues with a molecular mass of 98,759 Da. The deduced amino acid sequence showed homology to fungal and mammalian α-glucosidases and related enzymes, and the sequence around the putative active site was well conserved among these enzymes. The cloned gene was expressed in Escherichia coli cells to produce the α-glucosidase, which hydrolyzes not only maltose, but also soluble starch.

    Original languageEnglish
    Pages (from-to)500-505
    Number of pages6
    JournalJournal of biochemistry
    Volume119
    Issue number3
    DOIs
    Publication statusPublished - Mar 1996

    Keywords

    • Gene expression
    • Mucor javanicus
    • Nucleotide sequence
    • α-glucosidase

    ASJC Scopus subject areas

    • Biochemistry
    • Molecular Biology

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