Molecular cloning, sequencing, and expression of a cDNA encoding α-glucosidase from Mucor javanicus

Manabu Sugimoto, Yukio Suzuki

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18 Citations (Scopus)

Abstract

A cDNA encoding Mucor javanicus α-glucosidase was cloned and sequenced by the reverse transcription-polymerase chain reaction and rapid amplification of cDNA ends methods. The cDNA comprised 2,751 bp, and included an open reading frame which encodes a polypeptide of 864 amino acid residues with a molecular mass of 98,759 Da. The deduced amino acid sequence showed homology to fungal and mammalian α-glucosidases and related enzymes, and the sequence around the putative active site was well conserved among these enzymes. The cloned gene was expressed in Escherichia coli cells to produce the α-glucosidase, which hydrolyzes not only maltose, but also soluble starch.

Original languageEnglish
Pages (from-to)500-505
Number of pages6
JournalJournal of biochemistry
Volume119
Issue number3
DOIs
Publication statusPublished - Mar 1996

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Keywords

  • Gene expression
  • Mucor javanicus
  • Nucleotide sequence
  • α-glucosidase

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology

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