Molecular cloning, sequencing and characterization of the genes for adenosylcobalamin-dependent diol dehydratase of Klebsiella pneumoniae.

Takamasa Tobimatsu, M. Azuma, S. Hayashi, K. Nishimoto, T. Toraya

Research output: Contribution to journalArticle

14 Citations (Scopus)

Abstract

Klebsiella pneumoniae and some of the other Enterobacteriaceae form both diol dehydratase and glycerol dehydratase in response to growth substrates. To compare these enzymes produced by the same bacterium, the pdd genes of K. pneumoniae encoding adenosylcobalamin-dependent diol dehydratase were cloned and sequenced. The sequential three open reading frames (pddA, pddB, and pddC genes) encoded polypeptides of 554, 228, and 174 amino acid residues with predicted molecular weights of 60,379(alpha), 24,401(beta), and 19,489(gamma), respectively. The deduced amino acid sequences of the subunits were 84-100% and 54-71% identical with those reported for diol dehydratases and glycerol dehydratases, respectively.

Original languageEnglish
Pages (from-to)1774-1777
Number of pages4
JournalBioscience, Biotechnology and Biochemistry
Volume62
Issue number9
Publication statusPublished - Sep 1998

Fingerprint

Propanediol Dehydratase
glycerol dehydratase
Cloning
Klebsiella pneumoniae
Molecular Cloning
Glycerol
Amino acids
molecular cloning
Genes
glycerol
Polypeptides
Amino Acids
Bacteria
genes
Enzymes
Molecular weight
Enterobacteriaceae
Open Reading Frames
open reading frames
Amino Acid Sequence

ASJC Scopus subject areas

  • Bioengineering
  • Biotechnology
  • Biochemistry
  • Biochemistry, Genetics and Molecular Biology(all)
  • Chemistry (miscellaneous)
  • Applied Microbiology and Biotechnology
  • Food Science

Cite this

Molecular cloning, sequencing and characterization of the genes for adenosylcobalamin-dependent diol dehydratase of Klebsiella pneumoniae. / Tobimatsu, Takamasa; Azuma, M.; Hayashi, S.; Nishimoto, K.; Toraya, T.

In: Bioscience, Biotechnology and Biochemistry, Vol. 62, No. 9, 09.1998, p. 1774-1777.

Research output: Contribution to journalArticle

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