Molecular cloning of the S-layer protein gene of Campylobacter rectus ATCC 33238

M. Miyamoto, Y. Kobayashi, S. Kokeguchi, H. Ohta, H. Kurihara, K. Fukui, Y. Murayama

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

A genomic DNA library of Campylobacter rectus (Wolinella recta) ATCC 33238 was constructed using bacteriophage lambda EMBL3 as a vector. One clone expressing the S-layer protein was identified immunologically with the antiserum to the S-layer protein of C. rectus ATCC 33238. Western immunoblotting using monoclonal antibodies directed against the S-layer protein showed a single blot of recombinant protein at 150 kDa, suggesting that this clone contained the entire coding region of the S-layer protein gene. Additionally, the S-layer protein gene was subcloned into plasmid vector pUC18. Southern hybridization revealed that the S-layer protein gene was present on the chromosome of C. rectus as a single-copy gene, and that there were minor heterogeneities among the S-layer protein genes of clinical isolates. Moreover, a spontaneous stable mutant strain, which has no S-layer expression, also conserved the full-length gene of the S-layer protein.

Original languageEnglish
Pages (from-to)13-18
Number of pages6
JournalFEMS Microbiology Letters
Volume116
Issue number1
DOIs
Publication statusPublished - Feb 1 1994

Keywords

  • Campylobacter rectus
  • Gene cloning
  • S-layer

ASJC Scopus subject areas

  • Microbiology
  • Molecular Biology
  • Genetics

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