Molecular cloning of the S-layer protein gene of Campylobacter rectus ATCC 33238

M. Miyamoto, Y. Kobayashi, Susumu Kokeguchi, H. Ohta, H. Kurihara, K. Fukui, Y. Murayama

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

A genomic DNA library of Campylobacter rectus (Wolinella recta) ATCC 33238 was constructed using bacteriophage lambda EMBL3 as a vector. One clone expressing the S-layer protein was identified immunologically with the antiserum to the S-layer protein of C. rectus ATCC 33238. Western immunoblotting using monoclonal antibodies directed against the S-layer protein showed a single blot of recombinant protein at 150 kDa, suggesting that this clone contained the entire coding region of the S-layer protein gene. Additionally, the S-layer protein gene was subcloned into plasmid vector pUC18. Southern hybridization revealed that the S-layer protein gene was present on the chromosome of C. rectus as a single-copy gene, and that there were minor heterogeneities among the S-layer protein genes of clinical isolates. Moreover, a spontaneous stable mutant strain, which has no S-layer expression, also conserved the full-length gene of the S-layer protein.

Original languageEnglish
Pages (from-to)13-18
Number of pages6
JournalFEMS Microbiology Letters
Volume116
Issue number1
Publication statusPublished - Feb 1 1994

Fingerprint

Campylobacter rectus
Molecular Cloning
Genes
Wolinella
Clone Cells
Chromosomes, Human, 6-12 and X
Bacteriophage lambda
Genomic Library
S-layer proteins
Protein C
Gene Library
Recombinant Proteins
Rectum
Immune Sera
Plasmids
Western Blotting
Monoclonal Antibodies

Keywords

  • Campylobacter rectus
  • Gene cloning
  • S-layer

ASJC Scopus subject areas

  • Genetics
  • Molecular Biology
  • Applied Microbiology and Biotechnology
  • Microbiology

Cite this

Miyamoto, M., Kobayashi, Y., Kokeguchi, S., Ohta, H., Kurihara, H., Fukui, K., & Murayama, Y. (1994). Molecular cloning of the S-layer protein gene of Campylobacter rectus ATCC 33238. FEMS Microbiology Letters, 116(1), 13-18.

Molecular cloning of the S-layer protein gene of Campylobacter rectus ATCC 33238. / Miyamoto, M.; Kobayashi, Y.; Kokeguchi, Susumu; Ohta, H.; Kurihara, H.; Fukui, K.; Murayama, Y.

In: FEMS Microbiology Letters, Vol. 116, No. 1, 01.02.1994, p. 13-18.

Research output: Contribution to journalArticle

Miyamoto, M, Kobayashi, Y, Kokeguchi, S, Ohta, H, Kurihara, H, Fukui, K & Murayama, Y 1994, 'Molecular cloning of the S-layer protein gene of Campylobacter rectus ATCC 33238', FEMS Microbiology Letters, vol. 116, no. 1, pp. 13-18.
Miyamoto M, Kobayashi Y, Kokeguchi S, Ohta H, Kurihara H, Fukui K et al. Molecular cloning of the S-layer protein gene of Campylobacter rectus ATCC 33238. FEMS Microbiology Letters. 1994 Feb 1;116(1):13-18.
Miyamoto, M. ; Kobayashi, Y. ; Kokeguchi, Susumu ; Ohta, H. ; Kurihara, H. ; Fukui, K. ; Murayama, Y. / Molecular cloning of the S-layer protein gene of Campylobacter rectus ATCC 33238. In: FEMS Microbiology Letters. 1994 ; Vol. 116, No. 1. pp. 13-18.
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