Molecular cloning of the human hepatitis C virus genome from Japanese patients with non-A, non-B hepatitis

Nobuyuki Kato, Makoto Hijikata, Yuko Ootsuyama, Masanori Nakagawa, Showgo Ohkoshi, Takashi Sugimura, Kunitada Shimotohno

Research output: Contribution to journalArticle

1055 Citations (Scopus)

Abstract

The nucleotide sequence of the Japanese type of hepatitis C virus (HCV-J) genome, consisting of 9413 nucleotides, was determined by analyses of cDNA clones from plasma specimens from Japanese patients with chronic hepatitis. HCV-J genome contains a long open reading frame that can encode a sequence of 3010 amino acid residues. Comparison of HCV-J with the American isolate of HCV showed 22.6% difference in nucleotide sequence and 15.1% difference in amino acid sequence. Thus HCV-J and the American isolate of HCV are probably different subtypes of HCV. The relationship of HCV-J with other animal RNA virus families and the putative organization of the HCV-J genome are discussed. (.

Original languageEnglish
Pages (from-to)9524-9528
Number of pages5
JournalProceedings of the National Academy of Sciences of the United States of America
Volume87
Issue number24
Publication statusPublished - 1990
Externally publishedYes

Fingerprint

Molecular Cloning
Hepacivirus
Hepatitis
Genome
Amino Acid Sequence
RNA Viruses
Chronic Hepatitis
Open Reading Frames
Nucleotides
Complementary DNA
Clone Cells
Organizations
Viruses

Keywords

  • Gene structure
  • Nucleotide sequence
  • RNA virus

ASJC Scopus subject areas

  • Genetics
  • General

Cite this

Molecular cloning of the human hepatitis C virus genome from Japanese patients with non-A, non-B hepatitis. / Kato, Nobuyuki; Hijikata, Makoto; Ootsuyama, Yuko; Nakagawa, Masanori; Ohkoshi, Showgo; Sugimura, Takashi; Shimotohno, Kunitada.

In: Proceedings of the National Academy of Sciences of the United States of America, Vol. 87, No. 24, 1990, p. 9524-9528.

Research output: Contribution to journalArticle

Kato, Nobuyuki ; Hijikata, Makoto ; Ootsuyama, Yuko ; Nakagawa, Masanori ; Ohkoshi, Showgo ; Sugimura, Takashi ; Shimotohno, Kunitada. / Molecular cloning of the human hepatitis C virus genome from Japanese patients with non-A, non-B hepatitis. In: Proceedings of the National Academy of Sciences of the United States of America. 1990 ; Vol. 87, No. 24. pp. 9524-9528.
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