Molecular cloning of the fibroin light chain complementary DNA and its use in the study of the expression of the light chain gene in the posterior silk gland of Bombyx mori

K. Kimura, F. Oyama, Hitoshi Ueda, S. Mizuno, K. Shimura

Research output: Contribution to journalArticle

33 Citations (Scopus)

Abstract

Fibroin light chain (L-chain) mRNA (mol. wt 4.0×105 daltons) was purified from the posterior silk gland of the silkworm, Bombyx mori (J-131 strain). Double-stranded complementary DNA was synthesized and inserted into the PstI site of pBR322 employing the oligo(dC)-oligo(dG) tailing method. Several recombinant plasmids containing the inserts of about 800 base pairs were isolated. Hybridization-translation assay demonstrated that these clones hybridized specifically with the fibroin L-chain mRNA. One of these clones (pLA23) was used as a probe to investigate relative concentrations of the fibroin L-chain gene and mRNA in the posterior silk glands at different stages of late larval development.

Original languageEnglish
Pages (from-to)1167-1171
Number of pages5
JournalExperientia
Volume41
Issue number9
DOIs
Publication statusPublished - Sep 1985
Externally publishedYes

Fingerprint

Fibroins
Bombyx
Silk
Cloning
Molecular Cloning
Complementary DNA
Genes
Light
Messenger RNA
Clone Cells
Tailings
Base Pairing
Assays
Plasmids
DNA

Keywords

  • Bombyx mori
  • complementary DNA
  • fibroin light chain
  • molecular cloning
  • mRNA
  • silk gland
  • Silkworm

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)

Cite this

Molecular cloning of the fibroin light chain complementary DNA and its use in the study of the expression of the light chain gene in the posterior silk gland of Bombyx mori. / Kimura, K.; Oyama, F.; Ueda, Hitoshi; Mizuno, S.; Shimura, K.

In: Experientia, Vol. 41, No. 9, 09.1985, p. 1167-1171.

Research output: Contribution to journalArticle

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