Molecular cloning of the fibroin light chain complementary DNA and its use in the study of the expression of the light chain gene in the posterior silk gland of Bombyx mori

K. Kimura, F. Oyama, H. Ueda, S. Mizuno, K. Shimura

Research output: Contribution to journalArticle

34 Citations (Scopus)

Abstract

Fibroin light chain (L-chain) mRNA (mol. wt 4.0×105 daltons) was purified from the posterior silk gland of the silkworm, Bombyx mori (J-131 strain). Double-stranded complementary DNA was synthesized and inserted into the PstI site of pBR322 employing the oligo(dC)-oligo(dG) tailing method. Several recombinant plasmids containing the inserts of about 800 base pairs were isolated. Hybridization-translation assay demonstrated that these clones hybridized specifically with the fibroin L-chain mRNA. One of these clones (pLA23) was used as a probe to investigate relative concentrations of the fibroin L-chain gene and mRNA in the posterior silk glands at different stages of late larval development.

Original languageEnglish
Pages (from-to)1167-1171
Number of pages5
JournalExperientia
Volume41
Issue number9
DOIs
Publication statusPublished - Sep 1 1985
Externally publishedYes

Keywords

  • Bombyx mori
  • Silkworm
  • complementary DNA
  • fibroin light chain
  • mRNA
  • molecular cloning
  • silk gland

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)

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