Molecular cloning of the breakpoint of t(11;22)(q23;q11) chromosome translocation in an adult acute myelomonocytic leukaemia

Osamu Marukawa; Yukihiro Akao; Johji Inazawa; Takeshi Ariyama; Tatsuo Abe; Tomoki Naoe; Mitsune Tanimoto; Hidehiko Saito; Yoshinori Otsuki; Yoshihide Tsujimoto

doi:10.1046/j.1365-2141.1996.380916.x

Molecular cloning of the breakpoint of t(11;22)(q23;q11) chromosome translocation in an adult acute myelomonocytic leukaemia

Osamu Marukawa, Yukihiro Akao, Johji Inazawa, Takeshi Ariyama, Tatsuo Abe, Tomoki Naoe, Mitsune Tanimoto, Hidehiko Saito, Yoshinori Otsuki, Yoshihide Tsujimoto

Graduate School of Medicine Dentistry and Pharmaceutical Sciences

Research output: Contribution to journal › Article › peer-review

8 Citations (Scopus)

Abstract

Southern blot analysis with a cDNA probe of MLL indicated that the breakpoint is in a BamHI:8.3kb fragment which carries the exon 5-11 of MLL gene in DNA from an adult acute myelomonocytic leukaemia with a t(11;22)(q23,q11) translocation. The structural analysis of the rearranged MLL locus demonstrated that the breakpoint is localized between exon 8 and 9 of MLL locus. The normal counterpart fused to the MLL locus was proved to be derived from chromosome 22q11(AF-22) by somatic cell hybrids analysis and FISH. By FISH, AF-22 locus was localized to the region more centromeric to the BCR gene.

Original language	English
Pages (from-to)	687-691
Number of pages	5
Journal	British Journal of Haematology
Volume	92
Issue number	3
DOIs	https://doi.org/10.1046/j.1365-2141.1996.380916.x
Publication status	Published - 1996

Keywords

11q23
AF-22
Acute myelomonocytic leukaemia
MLL
t(11,22)(q23,q11)

ASJC Scopus subject areas

Hematology

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Molecular cloning of the breakpoint of t(11;22)(q23;q11) chromosome translocation in an adult acute myelomonocytic leukaemia. / Marukawa, Osamu; Akao, Yukihiro; Inazawa, Johji; Ariyama, Takeshi; Abe, Tatsuo; Naoe, Tomoki; Tanimoto, Mitsune; Saito, Hidehiko; Otsuki, Yoshinori; Tsujimoto, Yoshihide.

In: British Journal of Haematology, Vol. 92, No. 3, 1996, p. 687-691.

Research output: Contribution to journal › Article › peer-review

Marukawa, Osamu ; Akao, Yukihiro ; Inazawa, Johji ; Ariyama, Takeshi ; Abe, Tatsuo ; Naoe, Tomoki ; Tanimoto, Mitsune ; Saito, Hidehiko ; Otsuki, Yoshinori ; Tsujimoto, Yoshihide. / Molecular cloning of the breakpoint of t(11;22)(q23;q11) chromosome translocation in an adult acute myelomonocytic leukaemia. In: British Journal of Haematology. 1996 ; Vol. 92, No. 3. pp. 687-691.

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abstract = "Southern blot analysis with a cDNA probe of MLL indicated that the breakpoint is in a BamHI:8.3kb fragment which carries the exon 5-11 of MLL gene in DNA from an adult acute myelomonocytic leukaemia with a t(11;22)(q23,q11) translocation. The structural analysis of the rearranged MLL locus demonstrated that the breakpoint is localized between exon 8 and 9 of MLL locus. The normal counterpart fused to the MLL locus was proved to be derived from chromosome 22q11(AF-22) by somatic cell hybrids analysis and FISH. By FISH, AF-22 locus was localized to the region more centromeric to the BCR gene.",

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author = "Osamu Marukawa and Yukihiro Akao and Johji Inazawa and Takeshi Ariyama and Tatsuo Abe and Tomoki Naoe and Mitsune Tanimoto and Hidehiko Saito and Yoshinori Otsuki and Yoshihide Tsujimoto",

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AU - Marukawa, Osamu

AU - Akao, Yukihiro

AU - Inazawa, Johji

AU - Ariyama, Takeshi

AU - Abe, Tatsuo

AU - Naoe, Tomoki

AU - Tanimoto, Mitsune

AU - Saito, Hidehiko

AU - Otsuki, Yoshinori

AU - Tsujimoto, Yoshihide

PY - 1996

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AB - Southern blot analysis with a cDNA probe of MLL indicated that the breakpoint is in a BamHI:8.3kb fragment which carries the exon 5-11 of MLL gene in DNA from an adult acute myelomonocytic leukaemia with a t(11;22)(q23,q11) translocation. The structural analysis of the rearranged MLL locus demonstrated that the breakpoint is localized between exon 8 and 9 of MLL locus. The normal counterpart fused to the MLL locus was proved to be derived from chromosome 22q11(AF-22) by somatic cell hybrids analysis and FISH. By FISH, AF-22 locus was localized to the region more centromeric to the BCR gene.

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KW - t(11,22)(q23,q11)

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Molecular cloning of the breakpoint of t(11;22)(q23;q11) chromosome translocation in an adult acute myelomonocytic leukaemia

Abstract

Keywords

ASJC Scopus subject areas

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