Abstract
Southern blot analysis with a cDNA probe of MLL indicated that the breakpoint is in a BamHI:8.3kb fragment which carries the exon 5-11 of MLL gene in DNA from an adult acute myelomonocytic leukaemia with a t(11;22)(q23,q11) translocation. The structural analysis of the rearranged MLL locus demonstrated that the breakpoint is localized between exon 8 and 9 of MLL locus. The normal counterpart fused to the MLL locus was proved to be derived from chromosome 22q11(AF-22) by somatic cell hybrids analysis and FISH. By FISH, AF-22 locus was localized to the region more centromeric to the BCR gene.
| Original language | English |
|---|---|
| Pages (from-to) | 687-691 |
| Number of pages | 5 |
| Journal | British Journal of Haematology |
| Volume | 92 |
| Issue number | 3 |
| Publication status | Published - 1996 |
| Externally published | Yes |
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Keywords
- 11q23
- Acute myelomonocytic leukaemia
- AF-22
- MLL
- t(11,22)(q23,q11)
ASJC Scopus subject areas
- Hematology
Cite this
Molecular cloning of the breakpoint of t(11;22)(q23;q11) chromosome translocation in an adult acute myelomonocytic leukaemia. / Marukawa, Osamu; Akao, Yukihiro; Inazawa, Johji; Ariyama, Takeshi; Abe, Tatsuo; Naoe, Tomoki; Tanimoto, Mitsune; Saito, Hidehiko; Otsuki, Yoshinori; Tsujimoto, Yoshihide.
In: British Journal of Haematology, Vol. 92, No. 3, 1996, p. 687-691.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Molecular cloning of the breakpoint of t(11;22)(q23;q11) chromosome translocation in an adult acute myelomonocytic leukaemia
AU - Marukawa, Osamu
AU - Akao, Yukihiro
AU - Inazawa, Johji
AU - Ariyama, Takeshi
AU - Abe, Tatsuo
AU - Naoe, Tomoki
AU - Tanimoto, Mitsune
AU - Saito, Hidehiko
AU - Otsuki, Yoshinori
AU - Tsujimoto, Yoshihide
PY - 1996
Y1 - 1996
N2 - Southern blot analysis with a cDNA probe of MLL indicated that the breakpoint is in a BamHI:8.3kb fragment which carries the exon 5-11 of MLL gene in DNA from an adult acute myelomonocytic leukaemia with a t(11;22)(q23,q11) translocation. The structural analysis of the rearranged MLL locus demonstrated that the breakpoint is localized between exon 8 and 9 of MLL locus. The normal counterpart fused to the MLL locus was proved to be derived from chromosome 22q11(AF-22) by somatic cell hybrids analysis and FISH. By FISH, AF-22 locus was localized to the region more centromeric to the BCR gene.
AB - Southern blot analysis with a cDNA probe of MLL indicated that the breakpoint is in a BamHI:8.3kb fragment which carries the exon 5-11 of MLL gene in DNA from an adult acute myelomonocytic leukaemia with a t(11;22)(q23,q11) translocation. The structural analysis of the rearranged MLL locus demonstrated that the breakpoint is localized between exon 8 and 9 of MLL locus. The normal counterpart fused to the MLL locus was proved to be derived from chromosome 22q11(AF-22) by somatic cell hybrids analysis and FISH. By FISH, AF-22 locus was localized to the region more centromeric to the BCR gene.
KW - 11q23
KW - Acute myelomonocytic leukaemia
KW - AF-22
KW - MLL
KW - t(11,22)(q23,q11)
UR - http://www.scopus.com/inward/record.url?scp=9044246059&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=9044246059&partnerID=8YFLogxK
M3 - Article
C2 - 8616037
AN - SCOPUS:9044246059
VL - 92
SP - 687
EP - 691
JO - British Journal of Haematology
JF - British Journal of Haematology
SN - 0007-1048
IS - 3
ER -