Molecular cloning of the breakpoint of t(11;22)(q23;q11) chromosome translocation in an adult acute myelomonocytic leukaemia

Osamu Marukawa, Yukihiro Akao, Johji Inazawa, Takeshi Ariyama, Tatsuo Abe, Tomoki Naoe, Mitsune Tanimoto, Hidehiko Saito, Yoshinori Otsuki, Yoshihide Tsujimoto

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8 Citations (Scopus)

Abstract

Southern blot analysis with a cDNA probe of MLL indicated that the breakpoint is in a BamHI:8.3kb fragment which carries the exon 5-11 of MLL gene in DNA from an adult acute myelomonocytic leukaemia with a t(11;22)(q23,q11) translocation. The structural analysis of the rearranged MLL locus demonstrated that the breakpoint is localized between exon 8 and 9 of MLL locus. The normal counterpart fused to the MLL locus was proved to be derived from chromosome 22q11(AF-22) by somatic cell hybrids analysis and FISH. By FISH, AF-22 locus was localized to the region more centromeric to the BCR gene.

Original languageEnglish
Pages (from-to)687-691
Number of pages5
JournalBritish Journal of Haematology
Volume92
Issue number3
DOIs
Publication statusPublished - 1996

Keywords

  • 11q23
  • AF-22
  • Acute myelomonocytic leukaemia
  • MLL
  • t(11,22)(q23,q11)

ASJC Scopus subject areas

  • Hematology

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    Marukawa, O., Akao, Y., Inazawa, J., Ariyama, T., Abe, T., Naoe, T., Tanimoto, M., Saito, H., Otsuki, Y., & Tsujimoto, Y. (1996). Molecular cloning of the breakpoint of t(11;22)(q23;q11) chromosome translocation in an adult acute myelomonocytic leukaemia. British Journal of Haematology, 92(3), 687-691. https://doi.org/10.1046/j.1365-2141.1996.380916.x