Molecular cloning of the breakpoint of t(11;22)(q23;q11) chromosome translocation in an adult acute myelomonocytic leukaemia

Osamu Marukawa, Yukihiro Akao, Johji Inazawa, Takeshi Ariyama, Tatsuo Abe, Tomoki Naoe, Mitsune Tanimoto, Hidehiko Saito, Yoshinori Otsuki, Yoshihide Tsujimoto

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

Southern blot analysis with a cDNA probe of MLL indicated that the breakpoint is in a BamHI:8.3kb fragment which carries the exon 5-11 of MLL gene in DNA from an adult acute myelomonocytic leukaemia with a t(11;22)(q23,q11) translocation. The structural analysis of the rearranged MLL locus demonstrated that the breakpoint is localized between exon 8 and 9 of MLL locus. The normal counterpart fused to the MLL locus was proved to be derived from chromosome 22q11(AF-22) by somatic cell hybrids analysis and FISH. By FISH, AF-22 locus was localized to the region more centromeric to the BCR gene.

Original languageEnglish
Pages (from-to)687-691
Number of pages5
JournalBritish Journal of Haematology
Volume92
Issue number3
Publication statusPublished - 1996
Externally publishedYes

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Leukemia, Myelomonocytic, Acute
Molecular Cloning
Exons
Chromosomes
Hybrid Cells
Southern Blotting
Genes
Complementary DNA
DNA

Keywords

  • 11q23
  • Acute myelomonocytic leukaemia
  • AF-22
  • MLL
  • t(11,22)(q23,q11)

ASJC Scopus subject areas

  • Hematology

Cite this

Marukawa, O., Akao, Y., Inazawa, J., Ariyama, T., Abe, T., Naoe, T., ... Tsujimoto, Y. (1996). Molecular cloning of the breakpoint of t(11;22)(q23;q11) chromosome translocation in an adult acute myelomonocytic leukaemia. British Journal of Haematology, 92(3), 687-691.

Molecular cloning of the breakpoint of t(11;22)(q23;q11) chromosome translocation in an adult acute myelomonocytic leukaemia. / Marukawa, Osamu; Akao, Yukihiro; Inazawa, Johji; Ariyama, Takeshi; Abe, Tatsuo; Naoe, Tomoki; Tanimoto, Mitsune; Saito, Hidehiko; Otsuki, Yoshinori; Tsujimoto, Yoshihide.

In: British Journal of Haematology, Vol. 92, No. 3, 1996, p. 687-691.

Research output: Contribution to journalArticle

Marukawa, O, Akao, Y, Inazawa, J, Ariyama, T, Abe, T, Naoe, T, Tanimoto, M, Saito, H, Otsuki, Y & Tsujimoto, Y 1996, 'Molecular cloning of the breakpoint of t(11;22)(q23;q11) chromosome translocation in an adult acute myelomonocytic leukaemia', British Journal of Haematology, vol. 92, no. 3, pp. 687-691.
Marukawa, Osamu ; Akao, Yukihiro ; Inazawa, Johji ; Ariyama, Takeshi ; Abe, Tatsuo ; Naoe, Tomoki ; Tanimoto, Mitsune ; Saito, Hidehiko ; Otsuki, Yoshinori ; Tsujimoto, Yoshihide. / Molecular cloning of the breakpoint of t(11;22)(q23;q11) chromosome translocation in an adult acute myelomonocytic leukaemia. In: British Journal of Haematology. 1996 ; Vol. 92, No. 3. pp. 687-691.
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AU - Naoe, Tomoki

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