Molecular cloning of rabbit CAP-50, a calcyclin associated annexin protein

Hiroshi Tokumitsu, Akihiro Mizutani, Masa aki Muramatsu, Takashi Yokota, Ken ichi Arai, Hiroyoshi Hidaka

Research output: Contribution to journalArticle

18 Citations (Scopus)

Abstract

CAP-50 is a member of annexin family proteins which binds specifically to calcyclin in a Ca2+ dependent manner (Tokumitsu. H., Mizutani. A., Minami. H., Kobayashi. R., and Hidaka. H. (1992) J. Biol. Chem. 267,8919-8924). The cDNA representing the rabbit form of this protein has been cloned from rabbit lung cDNA library. Sequence analysis of two overlapping clones revealed a 81-nucleotides 5′-nontranslated region, 1512-nucleotides of open reading frame, a 672-nucleotides 3′-nontranslated region, and a poly(A) tail. Authenticity of the clones was confirmed by comparison of portions of the deduced amino acid sequence with eight sequences of proteolytic peptides obtained from rabbit lung protein. CAP-50 cDNA encodes a 503 residue protein with a calculatedMr of 54,043 and shows that the protein is composed of four imperfect repeats and hydrophobic N-terminal region. C-terminal region including four imperfect repeats shows 58.1% identity with human synexin (annexin VII), 48.0% identity with annexin I, 47.4% identity with annexin II, 60.1% identity with annexin IV, 54.5% identity with annexin V. Hydrophobic N-terminal region composed of 202 amino acid residues is not homologous with other annexin proteins suggesting that CAP-50 is a novel member of annexin family proteins.

Original languageEnglish
Pages (from-to)1227-1235
Number of pages9
JournalBiochemical and Biophysical Research Communications
Volume186
Issue number3
DOIs
Publication statusPublished - Aug 14 1992
Externally publishedYes

Fingerprint

Annexins
Cloning
Molecular Cloning
Rabbits
Annexin A7
Proteins
Nucleotides
Annexin A4
Complementary DNA
Clone Cells
Annexin A2
Annexin A1
Amino Acids
Lung
Annexin A5
calcyclin-associated protein 50
Gene Library
Open Reading Frames
Sequence Analysis
Amino Acid Sequence

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

Cite this

Molecular cloning of rabbit CAP-50, a calcyclin associated annexin protein. / Tokumitsu, Hiroshi; Mizutani, Akihiro; Muramatsu, Masa aki; Yokota, Takashi; Arai, Ken ichi; Hidaka, Hiroyoshi.

In: Biochemical and Biophysical Research Communications, Vol. 186, No. 3, 14.08.1992, p. 1227-1235.

Research output: Contribution to journalArticle

Tokumitsu, Hiroshi ; Mizutani, Akihiro ; Muramatsu, Masa aki ; Yokota, Takashi ; Arai, Ken ichi ; Hidaka, Hiroyoshi. / Molecular cloning of rabbit CAP-50, a calcyclin associated annexin protein. In: Biochemical and Biophysical Research Communications. 1992 ; Vol. 186, No. 3. pp. 1227-1235.
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