Molecular cloning of prophenoloxidase and the effects of dietary β-glucan and rutin on immune response in hemocytes of the fleshy shrimp, Fenneropenaeus chinensis

Sun Hye Bae, Bong Rae Kim, Bong Jung Kang, Naoaki Tsutsui, Tomoyuki Okutsu, Junpei Shinji, In Kwon Jang, Chang Hee Han, Marcy N. Wilder

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

To investigate the effects of dietary β-glucan (0.5 or 1 g kg-1 diet: 0.5-BG, 1-BG) and rutin (0.5 or 1 g kg-1 diet: 0.5-RT, 1-RT) after 10 days in the absence of pathogen challenge on the immune response of Fenneropenaeus chinensis, we determined total hemocyte count (THC) and the expression of four immune-related genes in hemocytes: those for prophenoloxidase (proPO), peroxinectin (PX), lipopolysaccharide and/or β-glucan binding protein (LGBP), and c-type lectin (CL). As a prerequisite for subsequent experiments, cDNA encoding proPO of the fleshy shrimp, Fenneropenaeus chinensis (f-proPO) was obtained from hemocytes; it had a full length of 3023bp, with an open reading frame (ORF) of 2061bp, a 105-bp 5'-untranslated region, and a 906-bp 3'-untranslated region containing the poly A signal. The THCs of shrimp fed β-glucan of 1 g kg-1 diet, and rutin of 1 g kg-1 diet were significantly higher than that of the control (P < 0.05). The expression of proPO mRNA was slightly downregulated and that of LGBP mRNA was upregulated (except in 1-RT). PX and CL mRNA remained constitutively expressed in all groups. Our results reveal that β-glucan and rutin dietary supplements have minimal effect on immune response in the absence of pathogen challenge.

Original languageEnglish
Pages (from-to)597-604
Number of pages8
JournalFish and Shellfish Immunology
Volume33
Issue number3
DOIs
Publication statusPublished - Sep 2012

Keywords

  • Fleshy shrimp
  • Prophenoloxidase
  • Rutin
  • Total hemocyte count
  • β-Glucan

ASJC Scopus subject areas

  • Environmental Chemistry
  • Aquatic Science

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