Molecular cloning of prophenoloxidase and the effects of dietary β-glucan and rutin on immune response in hemocytes of the fleshy shrimp, Fenneropenaeus chinensis

Sun Hye Bae, Bong Rae Kim, Bong Jung Kang, Naoaki Tsutsui, Tomoyuki Okutsu, Junpei Shinji, In Kwon Jang, Chang Hee Han, Marcy N. Wilder

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

To investigate the effects of dietary β-glucan (0.5 or 1 g kg-1 diet: 0.5-BG, 1-BG) and rutin (0.5 or 1 g kg-1 diet: 0.5-RT, 1-RT) after 10 days in the absence of pathogen challenge on the immune response of Fenneropenaeus chinensis, we determined total hemocyte count (THC) and the expression of four immune-related genes in hemocytes: those for prophenoloxidase (proPO), peroxinectin (PX), lipopolysaccharide and/or β-glucan binding protein (LGBP), and c-type lectin (CL). As a prerequisite for subsequent experiments, cDNA encoding proPO of the fleshy shrimp, Fenneropenaeus chinensis (f-proPO) was obtained from hemocytes; it had a full length of 3023bp, with an open reading frame (ORF) of 2061bp, a 105-bp 5'-untranslated region, and a 906-bp 3'-untranslated region containing the poly A signal. The THCs of shrimp fed β-glucan of 1 g kg-1 diet, and rutin of 1 g kg-1 diet were significantly higher than that of the control (P <0.05). The expression of proPO mRNA was slightly downregulated and that of LGBP mRNA was upregulated (except in 1-RT). PX and CL mRNA remained constitutively expressed in all groups. Our results reveal that β-glucan and rutin dietary supplements have minimal effect on immune response in the absence of pathogen challenge.

Original languageEnglish
Pages (from-to)597-604
Number of pages8
JournalFish and Shellfish Immunology
Volume33
Issue number3
DOIs
Publication statusPublished - Sep 2012
Externally publishedYes

Fingerprint

Fenneropenaeus chinensis
prophenoloxidase
Rutin
Glucans
Cloning
glucans
rutin
Nutrition
immune response
hemocytes
molecular cloning
shrimp
diet
Pathogens
Lectins
lectins
Messenger RNA
lipopolysaccharides
Lipopolysaccharides
binding proteins

Keywords

  • β-Glucan
  • Fleshy shrimp
  • Prophenoloxidase
  • Rutin
  • Total hemocyte count

ASJC Scopus subject areas

  • Aquatic Science
  • Environmental Chemistry

Cite this

Molecular cloning of prophenoloxidase and the effects of dietary β-glucan and rutin on immune response in hemocytes of the fleshy shrimp, Fenneropenaeus chinensis. / Bae, Sun Hye; Kim, Bong Rae; Kang, Bong Jung; Tsutsui, Naoaki; Okutsu, Tomoyuki; Shinji, Junpei; Jang, In Kwon; Han, Chang Hee; Wilder, Marcy N.

In: Fish and Shellfish Immunology, Vol. 33, No. 3, 09.2012, p. 597-604.

Research output: Contribution to journalArticle

Bae, Sun Hye ; Kim, Bong Rae ; Kang, Bong Jung ; Tsutsui, Naoaki ; Okutsu, Tomoyuki ; Shinji, Junpei ; Jang, In Kwon ; Han, Chang Hee ; Wilder, Marcy N. / Molecular cloning of prophenoloxidase and the effects of dietary β-glucan and rutin on immune response in hemocytes of the fleshy shrimp, Fenneropenaeus chinensis. In: Fish and Shellfish Immunology. 2012 ; Vol. 33, No. 3. pp. 597-604.
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T1 - Molecular cloning of prophenoloxidase and the effects of dietary β-glucan and rutin on immune response in hemocytes of the fleshy shrimp, Fenneropenaeus chinensis

AU - Bae, Sun Hye

AU - Kim, Bong Rae

AU - Kang, Bong Jung

AU - Tsutsui, Naoaki

AU - Okutsu, Tomoyuki

AU - Shinji, Junpei

AU - Jang, In Kwon

AU - Han, Chang Hee

AU - Wilder, Marcy N.

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AB - To investigate the effects of dietary β-glucan (0.5 or 1 g kg-1 diet: 0.5-BG, 1-BG) and rutin (0.5 or 1 g kg-1 diet: 0.5-RT, 1-RT) after 10 days in the absence of pathogen challenge on the immune response of Fenneropenaeus chinensis, we determined total hemocyte count (THC) and the expression of four immune-related genes in hemocytes: those for prophenoloxidase (proPO), peroxinectin (PX), lipopolysaccharide and/or β-glucan binding protein (LGBP), and c-type lectin (CL). As a prerequisite for subsequent experiments, cDNA encoding proPO of the fleshy shrimp, Fenneropenaeus chinensis (f-proPO) was obtained from hemocytes; it had a full length of 3023bp, with an open reading frame (ORF) of 2061bp, a 105-bp 5'-untranslated region, and a 906-bp 3'-untranslated region containing the poly A signal. The THCs of shrimp fed β-glucan of 1 g kg-1 diet, and rutin of 1 g kg-1 diet were significantly higher than that of the control (P <0.05). The expression of proPO mRNA was slightly downregulated and that of LGBP mRNA was upregulated (except in 1-RT). PX and CL mRNA remained constitutively expressed in all groups. Our results reveal that β-glucan and rutin dietary supplements have minimal effect on immune response in the absence of pathogen challenge.

KW - β-Glucan

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KW - Rutin

KW - Total hemocyte count

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