Molecular cloning of cDNA encoding the 16 KDa subunit of vacuolar H+-ATPase from mouse cerebellum

Hironori Hanada, Masahisa Hasebe, Yoshinori Moriyama, Masatomo Maeda, Masamitsu Futai

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Abstract

cDNa for the 16 kDa subunit of vacuolar H+-ATPase was cloned from mouse cerebellum and sequenced. The deduced polypeptide (155 amino acid residues; molecular weight, 15,808) was highly hydrophobic and homologous to the subunits of bovine adrenal medulla, Torpedo marmorata electric lobe, Drosophila and yeast. Glu-139 (supposed to be essential for proton transport) was also conserved as the potential dicyclohexylcarbodiimide binding site. The subunit had four transmembrane segments: Segment II and IV were highly homologous and Glu-139 was located in Segment IV. The roles of the non-conserved regions are discussed.

Original languageEnglish
Pages (from-to)1062-1067
Number of pages6
JournalBiochemical and Biophysical Research Communications
Volume176
Issue number3
DOIs
Publication statusPublished - May 15 1991

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ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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