Molecular cloning of cDNA and analysis of expression of the gene for α-glucosidase from the hypopharyngeal gland of the honeybee Apis mellifera L.

Kazuaki Ohashi, Miyuki Sawata, Hideaki Takeuchi, Shunji Natori, Takeo Kubo

Research output: Contribution to journalArticle

73 Citations (Scopus)

Abstract

Previously, we identified and purified an α-glucosidase with a molecular mass of 70 kDa from homogenate, of the hypopharyngeal gland of the older worker bee (forager bee) Apis mellifera L. Here, we isolated and sequenced a cDNA for the α-glucosidase. The cDNA encoded a protein consisting of 650 amino acids, which had high sequence identity with fruit fly and mosquito possible maltase gene products. RT-PCR showed that the gene was expressed specifically in the hypopharyngeal gland of the forager bee.

Original languageEnglish
Pages (from-to)380-385
Number of pages6
JournalBiochemical and Biophysical Research Communications
Volume221
Issue number2
DOIs
Publication statusPublished - Apr 16 1996
Externally publishedYes

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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